| HBV RNA复制子疫苗、DNA疫苗在BHK-21细胞中表达情况的比较 |
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| 引用本文： | 李莉,任林柱,张英,杨东,闫森,李小平,高飞,肖雪斌,欧阳红生,逄大欣. HBV RNA复制子疫苗、DNA疫苗在BHK-21细胞中表达情况的比较[J]. 中国农学通报, 2010, 26(10): 17-20 |
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| 作者姓名： | 李莉 任林柱 张英 杨东 闫森 李小平 高飞 肖雪斌 欧阳红生 逄大欣 |
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| 作者单位： | 吉林大学畜牧兽医学院,长春,130062 |
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| 摘 要： | 为建立更有效的乙肝疫苗,通过将所构建的HBVRNA疫苗和DNA疫苗分别转染BHK-21细胞,并比较其在细胞中表达情况及细胞凋亡现象。以pSFV为基础构建的疫苗载体的表达效率高于以pcDNA3.1为基础构建的疫苗载体,pSFV为基础构建的疫苗载体出现细胞凋亡的现象。HBVRNA疫苗比DNA疫苗表达效果更好,安全性更高,更具有应用前景。
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| 关 键 词： | 牡丹 牡丹 AM菌根 高温胁迫 生理生化 |
| 收稿时间： | 2009-12-10 |
| 修稿时间： | 2010-03-02 |
Detecting the expression efficiency of the RNA replicon vaccines and DNA vaccine of HBV in BHK-21 cells |
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| Li Li,Ren Linzhu,Zhang Ying,Yang Dong,Yan Sen,Li Xiaoping. Detecting the expression efficiency of the RNA replicon vaccines and DNA vaccine of HBV in BHK-21 cells[J]. Chinese Agricultural Science Bulletin, 2010, 26(10): 17-20 |
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| Authors: | Li Li Ren Linzhu Zhang Ying Yang Dong Yan Sen Li Xiaoping |
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| Affiliation: | College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062 |
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| Abstract: | Abstract: Construction of RNA replicon vaccine and DNA vaccine of HBV, then the 2 vectors were tranfected into BHK-21 cells and their expression efficiency was detected by ELISA with the kits used to detect HBsAg and preS2. Meantime, we found that the vectors based on pSFV can make BHK-21 cells dead (apoptosis). It is confirmed that the immune effect of RNA replicon vaccine is better than that of DNA vaccine. RNA vaccine is safer and more prospects. |
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| Keywords: | Apoptosiszz |
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