葵花籽对羊组织共轭亚油酸沉积及相关酶基因表达的影响

研究日粮中添加葵花籽(SS)对生长羊血液、不同组织共轭亚油酸(conjugated linoleic acid,CLA)的含量、硬脂酰辅酶A去饱和酶和脂肪酸合成酶基因表达的影响,选用18只健康体重相近3月龄生长羊(公)随机分为2组,分别饲喂不含葵花籽的基础日粮(对照组)和含6%葵花籽的日粮(处理组),进行为期30d的试验。结果表明:日粮中添加葵花籽极显著提高血液中t11-C18∶1和CLA-c9,t11的含量(P<0.01),处理组羊皮下脂肪组织中CLA-c9,t11含量极显著增加(P<0.01),肾周脂肪和肝脏组织中CLA-c9,t11含量有增加趋势,背最长肌CLA-c9,t11含量无显著变化(P>0.05)。葵花籽日粮能显著提高皮下脂肪SCD基因表达量(P<0.05),但对肝脏和肾周脂肪SCD基因表达量没有显著影响(P>0.05);处理组羊肝脏和肾周脂肪FAS基因表达量显著降低(P<0.05),皮下脂肪FAS基因表达量无显著变化(P>0.05)。

Effect of sunflower seed on conjugated linoleic acid accumulationand relative enzymes gene expression of sheep

The objective of this research is to study the effects of dietary sunflower seed(SS) on conjugated linoleic acid(CLA)content,Stearoyl-CoA desaturase(SCD) and fatty acid synthase(FAS)gene expression in different tissues of growing sheep.Eighteen 3-month old male growing sheeps((22.47 kg±0.62) kg) were randomly divided into two groups,where one group was fed basic dietary without SS(the Control Group) for 30 days,and the other group was fed experimental dietary with 6% SS(the Treatment Group) for 30 days.Results showed that supplementation of SS in dietary significantly increased the content of t11-C18:1,c9 and t11-CLA(P<0.01) in blood,and it also increased the content of c9 and t11-CLA in subcutaneus adipose tissue.The content of c9 and t11-CLA in perinephrit fat and liver had a growing tendency,while the content of c9 and t11-CLA in Longissimus dorsi muscles had no significant change.SCD gene expression of treatment group in subcutaneous fat increased 2.88 times(P<0.01),but there is no significantly change in liver and perinephrit fat(P>0.05).FAS gene expression of the treatment group in liver and perinephrit fat decreased significantly(P<0.05),while no significant change was observed in subcutaneous fat(P>0.05).