基于高通量测序的大麦In Del标记开发及应用

分子标记是遗传研究的基础工具,广泛应用于遗传多样性研究、种质鉴定、遗传图谱构建和基因定位等领域。本研究基于大麦的全基因组重测序数据鉴定出的二态性SNP位点,开发出遍布全基因组的118对InDel引物。以49份不同地理来源的大麦种质检测其有效性,筛选出2个等位基因的共显性InDel标记72对,进一步对288份大麦种质进行遗传距离分析及构建系统发育树。结果显示,筛选到32个有效性的核心InDel标记,覆盖大麦7条染色体上,平均PIC为0.44,平均MAF为0.34;基于InDel标记位点的供试大麦的系统发育树结果揭示供试大麦具有丰富的遗传多样性,且能够将具有相同地理来源的多数品种聚为一类。表明开发的32个二态性InDel标记可有效地用于鉴定品种间的亲缘关系,且丰富了大麦品种鉴定的分子标记。上述核心InDel标记在大麦品种鉴定、大麦资源亲缘关系分析以及群体划分方面具有一定的理论意义和应用价值。

Development and application of InDel markers based on high throughput sequencing in barley

Molecular markers are the basic tools in genetic research and widely used in genetic diversity analysis, germplasm identification, genetic map construction and gene mapping. Based on the whole genome re-sequencing data of barley, we developed 118 pairs of InDel markers covering the whole barley genome. Forty-nine barley germplasms from different geographical regions were used to detect the effectiveness of primers. And 72 co-dominant InDel markers with two alleles were selected for further genetic distance analysis in 288 barley germplasm and constructing phylogenetic tree. Thirty-two core InDel markers were screened, covering seven chromosomes of barley, with an average PIC of 0.44, and an average MAF of 0.34. The tested barley germplasms were rich in genetic diversity and could cluster most of the varieties with the same geographical origin. This study indicated that the 32 dimorphic InDel markers developed not only effectively reflect the genetic relationship between varieties, but also enrich the molecular markers used for the identification of barley varieties. The above core InDel markers have provided certain the theoretical significance and application value in on the identification of barley varieties, the relationship analysis of barley resources and group division of barley resources.