腹水综合征肉鸡空肠黏膜组织结构及相关基因表达分析

试验旨在研究肉鸡腹水综合征发生发展过程中空肠核因子κB （NF-κB）及紧密连接蛋白（闭合蛋白（occludin）、紧密连接蛋白-1（claudin-1）和胞质紧密黏连蛋白1（zonula occluden 1，ZO-1）） mRNA相对表达量，以及绒毛长度、隐窝深度和绒隐比的变化，为肉鸡腹水综合征的防治提供理论依据。选取40羽1日龄罗斯肉鸡常规饲养7 d后，随机分为对照组和模型组（饲料添加3%猪油和4%鱼粉，饮水添加0.12% NaCl，9~11℃低温饲养），35日龄检测体重，并取心脏组织测腹水心脏指数，取空肠组织以HE染色切片测量绒毛长度和隐窝深度，实时荧光定量PCR检测空肠NF-κB和紧密连接蛋白的基因表达量。结果表明，与对照组相比，模型组肉鸡体重极显著下降（P<0.01），腹水心脏指数极显著升高（P<0.01），空肠绒毛长度、绒隐比极显著下降（P<0.01），隐窝深度极显著升高（P<0.01），NF-κB mRNA相对表达量极显著升高（P<0.01），occludin、claudin-1和ZO-1 mRNA相对表达量极显著下降（P<0.01）。表明肠道黏膜结构与功能异常及肠通透性增高促进了肉鸡腹水综合征的发生发展。

Analysis of Histological Structure and Expression of Related Genes in Jejunum Mucosa of Broilers Ascites Syndrome

This experiment was aimed to study the relative expression of NF-κB and the tight junction protein(occludin,claudin-1 and ZO-1) mRNA and the changes of villus length (V),crypt depth (C) and V/C value in the development of broiler ascites syndrome,providing a theoretical basis for the prevention and treatment of broiler ascites syndrome.A total of 40 1-day-old Ross broilers were routinely reared for 7 days and randomly divided into control group and model group (added 3% lard and 4% fish meal to standard,added 0.12% NaCl to drinking water,and raising temperature at 9-11℃),and measured body weight at 35 days old.The ascites heart index was measured,and the jejunum tissue was taken for HE staining to measure the villus length and crypt depth.The gene expression of jejunal NF-κB and tight junction protein were detected by Real-time PCR.The results showed that compared with control group,the weight of broiler in model group was extremely significantly decreased (P<0.01),the ascites heart index was extremely significantly increased (P<0.01),the jejunum length and V/C value were extremely significantly decreased (P<0.01),the crypt depth was extremely significantly increased (P<0.01),the relative expression of NF-κB mRNA was extremely significantly increased (P<0.01),and the relative expression of tight junction proteins occludin,claudin-1 and ZO-1 mRNA were extremely significantly decreased (P<0.01).This results indicated that the intestinal mucosal structure and function abnormality and intestinal permeability increase promoted the development of broiler ascites syndrome.