猪PAANCR组织表达谱分析及其对脂质生成的影响

前期通过高通量测序比较去势和非去势猪皮下脂肪长链非编码RNA （long non-coding RNA，lncRNA）的表达差异，筛选到一个表达差异极显著的novel lncRNA——PAANCR，本试验旨在研究PAANCR在猪育肥不同阶段不同组织中的表达规律及其与脂质生成的关系。选用10头健康长×大二元杂交仔猪，在90和210 d各随机选取3头屠宰取样，使用实时荧光定量PCR方法检测PAANCR的表达谱，同时在3T3-L1细胞中使用siRNA干扰PAANCR的同源体lncRNA_{B130024G19Rik}，通过油红O染色和实时荧光定量PCR分析其对脂肪沉积的作用。结果发现，PAANCR具有明显的组织特异性和发育特异性，其在肺脏、子宫、脾脏、肾脏和脂肪组织中表达量较高，而在肝脏、小肠和脑中的表达量随着年龄增长而增加，在肌肉组织中几乎不表达。同时，通过siRNA介导的干扰能够显著抑制PAANCR同源体的表达量，PAANCR同源体表达量降低后，关键成脂分化和脂质沉积调控基因表达量也随之减少。提示PAANCR可以作为调控猪脂质生成的候选因子，对于深入研究lncRNA参与调控猪脂质生成的分子机制具有重要意义。

Expression Profile Analysis of Porcine PAANCR and Its Effect on Lipogenesis

In previous work,a novel lncRNA (PAANCR) was found to significantly associated with porcine lipogenesis,so the aim of this study was to investigate the expression of PAANCR in porcine different tissues and fatten periods and its relationship with lipogenesis.10 healthy Landrace×Large White piglets were selected,3 pigs were randomly selected and slaughtered at 90 and 210 days,respectively.Real-time PCR was used to detected the expression files of PAANCR,meanwhile Oil Red O staining and Real-time PCR were used to analyze lncRNA_{B130024G19Rik}'s (a mouse lncRNA,the homologue of PAANCR) effect on lipid deposition in 3T3-L1 preadipocyte.The results showed that the expression trends of PAANCR was tissue- and development-specific,its expressional level was higher in lung,uterus,spleen,kidney and adipose tissues,its expression levels was up-regulated in liver,small intestine and brain during the development,which couldn't be detected in muscle.During differentiation,when the expression of lncRNA_{B130024G19Rik} was knockdown,the expression level of genes associated with adipogenesis and lipid deposition were down-regulated.The results indicated that PAANCR could be used as candidate factor for porcine lipogenesis,and it was meaningful for understanding the molecular mechanism of lncRNA on lipogenesis.