miR-140-3p inhibits viability,invasion and migration of non-small-cell lung cancer A549 cells by targeting PD-L1 |
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Authors: | ZHANG Yu-xuan LI Chun-wei MAO Wen-hao ZHU Ke-yan SHAO Yang-qian DENG Xiao-ming |
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Affiliation: | 1. Department of Combination of TCM and Western Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China;2. Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China;3. Department of Thyroid, Head and Neck Surgery, Henan Cancer Hospital, Zhengzhou 450008, China |
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Abstract: | AIM: To explore the target relationship between microRNA-140-3p (miR-140-3p) and programmed cell death ligand 1 (PD-L1) and their effect on the viability, migration and invasion of non-small-cell lung cancer A549 cells.METHODS: RT-qPCR was used to detect the miR-140-3p expression in HLF-1, A549 and H1299 cells, and then the A549 cells with the most significant difference were selected as the subsequent research object. TargetScan software and dual-luciferase reporter assay were performed to predict and confirm the target relationship between miR-140-3p and PD-L1. RT-qPCR and Western blot were used to determine the effects of miR-140-3p mimic and inhibitor on PD-L1 expression level. MTT assay was used to detect the viability of A549 cells. Transwell assay was performed to detect the migration and invasion abilities of the A549 cells.RESULTS: miR-140-3p was significantly down-regulated in the A549 cells and H1299 cells (P<0.05). Transfection with miR-140-3p mimic decreased the expression of PD-L1 and inhibited the viability, migration and invasion of the A549 cells. Transfection with pcDNA3.0-PD-L1 reversed the inhibitory effect of miR-140-3p on the viability, migration and invasion of the A549 cells.CONCLUSION: miR-140-3p inhibits the viability, migration and invasion of A549 cells by targeting PD-L1. |
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Keywords: | MicroRNA-140-3p Programmed cell death ligand 1 Non-small-cell lung cancer |
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