Galectin-9 regulates SHH signaling pathway to influence apoptosis of colorectal cancer HT29 cells |
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Authors: | ZHAO Xin TANG Ya-ping CHEN Lin-lin WANG Chun-yue YE Feng |
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Institution: | 1. Department of Oncology, The First Affiliated Hospital of Xiamen University, Xiamen 361000, China;
2. Department of Digestive Medicine, Changsha 410008, China;
3. Department of Hepatobiliary Surgery, Xiangya Hospital of Central South University, Changsha 410008, China |
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Abstract: | AIM: To investigate the effect of galectin-9 on the apoptosis of colorectal cancer HT29 cells. METHODS: Galectin-9 over-expression vector (pcDNA3.1-Galectin-9) or control vector (pcDNA3.1) was transfected into the HT29 cells. The galectin-9 over-expression was detected by real-time PCR and Western blot. Annexin V-FITC/PI double staining was used to detect the apoptosis. The protein level of activated caspase-3 and the expression of SHH signaling pathway-related proteins Smo, Gli1 and SHH in the HT29 cells were determined by Western blot. SHH signaling pathway specific inhibitor cyclopamine was used to treat the HT29 cells with up-regulated galectin-9 expression, and the apoptosis, the protein level of cleaved caspase-3 and the expression of SHH signaling pathway-related proteins Smo, Gli1 and SHH in the HT29 cells were detected by the above methods. RESULTS: Transfection with pcDNA3.1-Galectin-9 up-regulated galectin-9 expression at mRNA and protein levels in the colorectal cancer HT29 cells (P<0.05). The apoptotic rate of the HT29 cells was increased after galectin-9 up-regulation. The protein level of cleaved caspase-3 in the cells was increased, while the expression levels of SHH signaling pathway-related proteins Smo, Gli1 and SHH were decreased. Cyclopamine treatment further induced the apoptosis of the HT29 cells with up-regulation of galectin-9, increased the protein le-vels of cleaved caspase-3, and decreased the activation level of SHH signaling pathway in the HT29 cells (P<0.05). CONCLUSION: Galectin-9 induces the apoptosis of colorectal cancer HT29 cells by inhibiting SHH signaling pathway. |
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Keywords: | Colorectal cancer Galectin-9 Apoptosis SHH signaling pathway |
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