日粮中添加凝结芽孢杆菌对断奶仔猪小肠功能的影响

本研究旨在探究日粮中添加凝结芽孢杆菌(BC)对断奶仔猪小肠功能的影响。试验选取平均体重为(6.5±0.21) kg的21日龄三元杂交(杜×长×大)断奶仔猪24头,随机分为3组,每组8个重复,每个重复1头猪。对照组饲喂基础日粮,BCⅠ、BCⅡ组分别在基础日粮中添加2×10~6、2×10~7 CFU/g凝结芽孢杆菌。于试验第21天,所有仔猪灌服D-木糖(0.1 g/(kg·BW)),1 h后采血,屠宰,取回肠黏膜样品及回肠、结肠食糜。结果表明:与对照组相比,BCⅠ、BCⅡ组血浆二胺氧化酶(DAO)活力显著降低(P<0.05),BCⅠ组血浆D-木糖含量显著增加(P<0.05);BCⅠ、BCⅡ组回肠谷胱甘肽过氧化物酶(GSH-Px)的活力显著增加(P<0.05),BCⅡ组回肠谷胱甘肽(GSH)含量增加(P>0.05),BCⅠ组回肠丙二醛(MDA)含量显著降低(P<0.05);BCⅠ组回肠黏膜水通道蛋白3(AQP3)、碱性氨基酸转运载体(b^0,+AT)、钠葡萄糖共转运载体1(SGLT-1)的mRNA表达量均显著增加(P<0.05),BCⅡ组回肠黏膜AQP3、AQP10、内向整流钾离子通道13(KCNJ13)、b^0,+AT、SGLT-1的mRNA表达量均显著增加(P<0.05);BCⅠ组回肠及结肠肠球菌、双歧杆菌数量均显著升高(P<0.05),BCⅡ组回肠肠球菌及结肠肠球菌及双歧杆菌数量均显著升高(P<0.05)。综合试验结果,日粮中添加凝结芽孢杆菌可提高断奶仔猪小肠道吸收转运能力及抗氧化能力,改善断奶仔猪小肠内菌群结构。

Effects of Bacillus coagulans on Intestinal Function in Weaned Piglets

The study was to investigate the effects of Bacillus coagulans (BC) on intestinal function in weaned piglets.Twenty-four piglets were randomly allocated into three groups.The control group was fed the based diet,whereas the BC Ⅰ and BC Ⅱ groups were fed the basal diet supplemented with 2×10⁶ and 2×10⁷ CFU/g Bacillus coagulans,respectively.On the 21th day of the trial,D-xylose (0.1 g/(kg·BW)) was orally administrated to all piglets.One hour later,jugular vein blood samples were collected,and then all pigs were sacrificed to obtain the mid-ileum and the chyme of ileum and colon.The results showed that,cmpared with the control group,plasma diamine oxidase (DAO) activities in BC Ⅰ and BC Ⅱ groups were significantly decreased (P<0.05),and plasma D-xylose content was significantly increased in BC Ⅰ group (P<0.05).The actitivies of ileum glutathione peroxidase (GSH-Px) in BC Ⅰ and BC Ⅱ groups increased significantly (P<0.05),and the content of ileal glutathione (GSH) in BC Ⅱ group increased (P>0.05).The content of ileal malondialdehyde (MDA) was significantly decreased (P<0.05);The mRNA expression of ileal mucosa aquaporin 3 (AQP3),basic amino acid transporter (b^0,+AT) and sodium glucose cotransporter 1 (SGLT-1) in BC Ⅰ group increased significantly (P<0.05).The mRNA expression levels of ileum mucosa AQP3,AQP10,inward rectifier potassium channel 13 (KCNJ13),b^0,+AT,SGLT-1 were significantly increased in BC Ⅱ group (P<0.05).The number of Enterococcus,Bifidobacterium in the ileum and colon of BC Ⅰ group were significantly increased (P<0.05).The number of Enterococcus in the ileum,Enterococcus,Bifidobacterium in colon of BC Ⅱ group were significantly increased (P<0.05).In summary,the addition of Bacillus coagulans in the diet could improve the intestinal absorption,transport capacity and antioxidant capacity of weaned piglets,and improve the intestinal flora structure of weaned piglets.