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| 鸭源多杀性巴氏杆菌对磺胺类药物的耐药性检测及耐药基因分析 | |
| 引用本文： | 陈红梅,程龙飞,邓辉,刘荣昌,傅光华,施少华,傅秋玲,万春和,郑庆礼,黄瑜,黄小红. 鸭源多杀性巴氏杆菌对磺胺类药物的耐药性检测及耐药基因分析[J]. 中国畜牧兽医, 2019, 46(1): 223-230. DOI: 10.16431/j.cnki.1671-7236.2019.01.026 |
| 作者姓名： | 陈红梅 程龙飞 邓辉 刘荣昌 傅光华 施少华 傅秋玲 万春和 郑庆礼 黄瑜 黄小红 |
| 作者单位： | 1. 福建农林大学生命科学学院, 福州 350002; 2. 福建省农业科学院畜牧兽医研究所, 福建省畜禽疫病防治工程技术研究中心, 福建省禽病防治重点实验室, 福州 350013; 3. 福建农林大学动物科学学院, 中西兽医结合与动物保健福建省高校重点实验室, 福州 350002 |
| 基金项目： | 现代农业产业技术体系建设专项资金（CARS-42-18）；福建省属公益类科研院所基本科研项目（2016R1022-2）；福建省农业科学院畜牧兽医创新团队（STIT2017-1-5）；福建省教育厅实验室开放基金项目 |
| 摘 要： | 为明确鸭源多杀性巴氏杆菌对磺胺类药物的耐药性及耐药基因的分布特征,试验采用琼脂二倍稀释法对分离自福建、广东和江西等地的89株鸭源多杀性巴氏杆菌进行甲氧苄啶/磺胺甲噁唑的耐受性检测,用PCR法检测各耐药菌株的磺胺类耐药基因(sul1、sul2和sul3),并利用多位点序列分型(MLST)分析耐药菌株间的亲缘关系。结果显示,33株鸭源多杀性巴氏杆菌对甲氧苄啶/磺胺甲噁唑耐受,耐药率为37.1%(33/89);在耐药菌株中,sul1、sul2和sul3基因检出率分别为100%(33/33)、97.0%(32/33)和21.2%(7/33),其中sul1和sul2基因同时检出率为97.0%(32/33),sul1、sul2和sul3基因同时检出率为21.2%(7/33);所有耐药菌株均属于ST129型。以上结果表明,鸭源多杀性巴氏杆菌对磺胺类药物具有一定的耐药性,其中的耐药菌株都属于同一序列型ST129,均携带有sul1耐药基因,且绝大多数耐药菌株同时携带sul1与sul2两种耐药基因,提示在福建、广东和江西等地需慎用磺胺类药物来防治该细菌的感染。 |
| 关 键 词： | 多杀性巴氏杆菌 磺胺类药物 多位点序列分型 sul1基因 sul2基因 sul3基因 |
| 收稿时间： | 2018-05-11 |
Detection of Sulfonamides Sensitivity and Analysis of Resistant Genes of Pasteurella multocida Isolated from Ducks |
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| CHEN Hongmei,CHENG Longfei,DENG Hui,LIU Rongchang,FU Guanghua,SHI Shaohua,FU Qiuling,WAN Chunhe,ZHENG Qingli,HUANG Yu,HUANG Xiaohong. Detection of Sulfonamides Sensitivity and Analysis of Resistant Genes of Pasteurella multocida Isolated from Ducks[J]. China Animal Husbandry & Veterinary Medicine, 2019, 46(1): 223-230. DOI: 10.16431/j.cnki.1671-7236.2019.01.026 | |
| Authors: | CHEN Hongmei CHENG Longfei DENG Hui LIU Rongchang FU Guanghua SHI Shaohua FU Qiuling WAN Chunhe ZHENG Qingli HUANG Yu HUANG Xiaohong |
| Affiliation: | (College of Life Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fujian Animal Diseases Control Technology Center,Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention,Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China;University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province,College of Animal Science and Technology,Fujian Agriculture and Forestry University,Fuzhou 350002,China) |
| Abstract: | In order to understand the antimicrobial sensitibity to sulfonamides and distribution of sulfonamides resistance genes of Pasteurella multocida strains isolated from ducks in Fujian,Guangdong,Jiangxi,etc,89 Pasteurella multocida strains were tested for antimicrobial susceptibility to trimethoprim-sulfamethoxazole by agar dilution method, PCR method and multi-locus sequence typing (MLST) were used for detection of sulfonamides resistance genes and to analyze the genetic relationship among the sulfonamides-resistant strains.The results showed that 33 strains (37.1%) were resistant to trimethoprim-sulfamethoxazole.Among the 33 sulfonamides-resistant strains,the detection rates of sul1,sul2 and sul3 genes were 100%(33/33),97.0%(32/33) and 21.2%(7/33).The simultaneous detection rate of sul1 and sul2 genes were 97.0%(32/33).The simultaneous detection rate of sul1,sul2 and sul3 genes were 21.2%(7/33).All of the 33 sulfonamides-resistant strains belonged to the ST129 sequence type.The results indicated that there was sulfonamides resistance to Pasteurella multocida isolated from ducks,and all the sulfonamides-resistant strains,which belonged to the same sequence type ST129,carried sul1 resistant gene,most of sulfonamides-resistant strains carried sul1 and sul2 resistant genes,suggesting that it should be rationally used sulfonamides to prevent and cure the infection of Pasteurella multocida isolated from ducks in some areas of Fujian,Guangdong and Jiangxi,etc. |
| Keywords: | Pasteurella multocida sulfonamides MLST sul1 gene sul2 gene sul3 gene |
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