SDF-1α/CXCR4 axis promotes migration and invasion of pancreatic cancer cells through inducing epithelial-mesenchymal transition |
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Authors: | LI Ruo-meng ZOU Jin-mao LI Ya-qing CHEN Shao-jie LIAN Guo-da CHEN Yin-ting SU Hong HUANG Kai-hong |
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Institution: | Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Department of Gastroenterology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China |
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Abstract: | AIM:To investigate the role of SDF-1α/CXCR4 axis in pancreatic cancer cell migration and invasion.METHODS:The mRNA expression of CXCR4 in 4 pancreatic cancer cell lines was detected by RT-qPCR. The migration and invasion abilities of PANC-1 cells with the axis activated by exogenous SDF-1α or inhibited by CXCR4 inhibitor AMD3100 were detected by Transwell assays. The cell viability was measured by MTS assay. The protein expression of the epithelial-mesenchymal transition (EMT)-related molecules in the cells treated with exogenous SDF-1α or AMD3100 was determined by Western blot.RESULTS:All of the 4 pancreatic cancer cell lines expressed CXCR4 mRNA, while the PANC-1 cell line expressed the most. Exogenous SDF-1α promoted the migration and invasion abilities of PANC-1 cells, which was inhibited by AMD3100. The PANC-1 cells treated with exogenous SDF-1α for 72 h grew faster, while SDF-1α combined with AMD3100 made little significance to the viability of PANC-1 cells. Exogenous SDF-1α induced EMT of PANC-1 cells by up-regulating the expression of SNAIL and TWIST, and AMD3100 reversed this effect.CONCLUSION:SDF-1α/CXCR4 axis enhances the migration and invasion abilities of pancreatic cancer cells through inducing EMT. |
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Keywords: | Pancreatic cancer SDF-1/CXCR4 axis Epithelial-mesenchymal transition |
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