Anthocyanins attenuates hepatic fibrosis and inflammation in non-alcoho-lic fatty liver disease mice |
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Authors: | YANG Jiang-xia BAI Jian-ying LI Fu-ping ZHANG Xiu-fen XU Zhao-juan ZHAO Dong |
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Affiliation: | 1. Experimental Center, Hebei University of Chinese Medicine, Shijiazhuang 050200, China;2. School of Nursing, Hebei University of Chinese Medicine, Shijiazhuang 050200, China;3. Office of Educational Administration, Hebei University of Chinese Medicine, Shijiazhuang 050200, China |
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Abstract: | AIM:To explore the therapeutic effect of anthocyanins from Fructus Acanthophorae on high-fat diet-induced non-alcoholic fatty liver disease (NAFLD) in mice and the potential mechanism. METHODS:NAFLD mouse model was established by high-fat diet, and interferred with anthocyanins. The liver weight, and serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC) and low-density li-poprotein cholesterol (LDL-C) were measured. The liver tissues were staining with HE, Oil Red O and Masson's trichrome. The protein levels of inflammatory factors tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6 and IL-10 in the liver tissues were determined by Western blot. The liver macrophage, white blood cell and mononuclear cell infiltration was detected by immunohistochemical method. The chemokines CCL7 and MCP-1 were also measured by immunohistochemical method. RESULTS:Anthocyanins significantly inhibited the increases in the liver weight, ALT, AST, TG, TC and LDL-C induced by high-fat diet. Anthocyanins attenuated the liver fibrosis and inflammatory cell infiltration caused by high-fat diet, and reduced the levels of inflammatory factors TNF-α, IL-1β, IL-6, IL-10 and inflammatory chemokines CCL7 and MCP-1 in the liver tissues. CONCLUSION:Anthocyanins significantly alleviate non-alcoholic fatty liver disease caused by high-fat diet though reducing inflammatory factors, inflammatory cell infiltration and inflammatory chemokines. |
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Keywords: | Anthocyanins Non-alcoholic fatty liver disease Inflammation Fibrosis |
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