Effects of propofol on expression of CXCR4/CXCR7 and migration ability of human breast cancer MCF-7 cells in vitro |
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| Authors: | YANG Tao-bo YI Han SONG Juan CHEN Fei ZHU Ying WANG Shou-yong |
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| Affiliation: | Department of Anesthesiology, Key Laboratory of Child Development and Disorders of Ministry of Education, China International Science and Technology Cooperation, Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Pediatrics, Children Hospital of Chongqing Medical University, Chongqing 400014, China |
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| Abstract: | AIM:To investigate the effects of propofol on the expression of CXC-chemokine receptor (CXCR)4/CXCR7 and migration ability of human breast cancer MCF-7 cells in vitro. METHODS:MCF-7 cells were randomly divided into 4 groups,control group, lipid emulsion group, 3 mg/L and 8 mg/L propofol group. The cell viability was measured by MTT assay. The migration ability was detected by wound-healing assay and Transwell assay. The mRNA level of CXCR4/CXCR7 was detected by RT-qPCR. The protein expression leve of CXCR4/CXCR7 was determined by Western blot. RESULTS:Compared with control group, the scratching healing rates in 3 and 8 mg/L propofol group were decreased (P<0.05), and the chemotactic index was also decreased (P<0.05). The protein expression level of CXCR4/CXCR7 was decreased in 3 and 8 mg/L propofol group(P<0.05). However, both the mRNA level of CXCR4/CXCR7 and the viability of the MCF-7 cells kept no change. CONCLUSION:Propofol down-regulates the protein expression of CXCR4/CXCR7 and inhibits the migration ability of breast cancer MCF-7 cells in vitro. |
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| Keywords: | Propofol Breast cancer CXCR4/CXCR7 Cell migration |
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