2013—2018年广西地区猪伪狂犬病病毒gB、gE与TK基因遗传变异分析

为了解广西近年猪伪狂犬病（PR）流行情况及猪伪狂犬病病毒（PRV）流行株gB、gE、TK基因的遗传变异特点，于2013-2018年采集广西各地区疑似PR发病猪靶组织714份，进行PRV的PCR检测及病毒分离鉴定，并运用DNAStar、MEGA 6.0等生物学软件对分离毒株gB、gE、TK基因进行遗传变异分析。结果显示：样品检测阳性率为24.5%（175/714），共分离获得PRV流行株38株。受检猪群普遍存在PRV感染，经典毒株及变异毒株并存，而且以变异毒株为主。广西流行株不同亚群PRV在gB、gE、TK基因上均存在相同的氨基酸变异特点，与国内变异株亲缘关系较近。推测广西流行株存在两种不同重组变异形式产生的PRV重组毒株，分别为疫苗株gB基因和变异株gE基因重组产生的重组毒株，以及经典株的TK基因被疫苗株（HB98）的TK基因替换而产生的重组毒株。近年广西受检猪场较普遍存在PRV感染，而且PRV流行株以变异毒株为主，可能存在两种不同重组变异形式产生的重组毒株，猪场需重视及实施针对性PR免疫防控计划。

Genetic Variation Analysis of gB,gE and TK Genes of Pseudorabies Virus in Guangxi from 2013 to 2018

To study the epidemic situation of pseudorabies (PR) and genetic variation of epidemic strains of pseudorabies virus(PRV)in Guangxi from 2013 to 2018, 714 target tissues of suspected PR-infected pigs in various regions of Guangxi were collected and tested by PCR and virus isolation. gB, gE and TK genes of isolated strains were amplified, cloned, sequenced and analyzed by DNAStar and MEGA 6.0. The results indicated that the positive rate of suspected tissues was 24.5% (175/714), and 38 PRV strains were isolated. PRV infection of Pig is common in the tested herds. There co-existed classical strains and the variant strains, and the mutant strains are dominant. gB, gE and TK genes of different subtypes of isolated strains had the same amino acid variation characteristics and had closer relationship with domestic mutant strains. It is speculated that there were two different recombinant forms of PRV strains in Guangxi. The first was recombinant by vaccine strains (gB gene) and mutant strains (gE gene). The other one, classical strain (TK gene) was replaced by vaccine strains (TK gene). In recent years, PRV infection was common in the pig farms in Guangxi, and the epidemic strains were mainly mutant strains. And there may be two PRV recombinant forms of recombinant variant strains. Pig farms should pay more attention to practice PR targeted immunity plan.