Regulatory effect of astragaloside IV on SDF-1α/CXCR4 in EPCs |
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Authors: | YANG Lei LIU Ping LIU Nuan ZHAO Wei TAO Ling-ling LI Xing MAO Bing-yu |
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Institution: | Henan Key Laboratory of Zhang ZhongJing Formulae and Herbs for Immunoregulation, Zhang ZhongJing School of Chinese Medicine, Nanyang Institute of Technology, Nanyang 473004, China |
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Abstract: | AIM: To investigate the effects of astragaloside IV (AS-IV) on chemokine receptor 4 (CXCR4) and stromal cell-derived factor 1α (SDF-1α) in endothelial progenitor cells (EPCs) and its mechanism. METHODS: Rat bone marrow-derived EPCs were cultured in vitro. The proliferation, adhesion, migration, apoptosis and tube formation capacity of EPCs treated with AS-IV and AMD3100, a specific blocker of CXCR4, were observed. The effects of AS-IV on the expression of SDF-1α/CXCR4 at mRNA and protein levels and the protein level of p-CXCR4 in the EPCs were determined. RESULTS: AS-IV significantly enhanced the proliferation, adhesion, migration and tube formation abilities of EPCs, reduced the apoptosis of EPCs, and up-regulated the mRNA and protein expression of SDF-1α and CXCR4 and the p-CXCR4 protein level in the EPCs. On the other hand, AMD3100 blocked the up-regulating effect of AS-IV on the mRNA and protein expression of CXCR4 and the p-CXCR4 protein level in the EPCs, but did not affect the effect of AS-IV on the expression of SDF-1α. CONCLUSION: AS-IV might enhance the biological function of EPCs by regulating the expression of SDF-1α/CXCR in EPCs. |
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Keywords: | Astragaloside IV Endothelial progenitor cells CXC chemokine receptor 4 Stromal cell-derived factor 1α Angiogenesis |
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