CREB-H基因在猪不同组织中表达谱及肝脏中的表达规律

本研究旨在分析环腺苷酸应答元件结合蛋白H（cyclic AMP-responsive element-binding protein 3-like 3,CREB-H）在猪不同组织中的表达谱及其在马身猪和大白猪肝脏中的发育性表达规律。采用实时荧光定量PCR和Western blotting技术检测1日龄猪12个组织（心脏、肝脏、脾脏、肺脏、肾脏、胃、小肠、小脑、下丘脑、背最长肌、股肌和腰肌）中CREB-H基因的表达谱,以及CREB-H在1、30、60、90、120、150和180日龄马身猪和大白猪肝脏中的表达规律。结果显示,CREB-H基因mRNA在马身猪的12个组织中广泛表达,其中在肝脏和小肠中高表达;CREB-H蛋白在肝脏组织中的表达量显著高于其他组织（P<0.05）,在心脏、脾脏和小脑中不表达。猪肝脏CREB-H基因mRNA和蛋白的发育表达受日龄、品种、品种与日龄相互作用的影响（P<0.01）。马身猪和大白猪肝脏中CREB-H基因mRNA和蛋白的表达量均在1日龄时达到最大值。在各发育阶段,马身猪CREB-H蛋白的表达量均极显著高于大白猪（P<0.01）,且CREB-H主要在猪肝脏中表达。CREB-H在两猪种肝脏中的表达存在时空差异,可能与猪在不同发育期的脂质代谢能力有关,本试验结果为研究猪的脂质代谢调控机制提供一定的理论依据。

Expression Profile and Patterns of CREB-H Gene in Different Tissues and Liver of Pig

The aim of this study was to investigate the expression characteristics of cyclic AMP-responsive elmment-binding protein 3-like 3 (CREB-H) in different tissues of pig and the expression patterns of CREB-H in liver of Mashen and Large White pigs.Real-time quantitative PCR and Western blotting were used to detect the expression profile of CREB-H gene in 12 tissues (heart,liver,spleen,lung,kidney,stomach,small intestine,cerebellum,hypothalamus,longissimus dorsi muscle,biceps femoris and psoas major) of Mashen pig and the expression patterns of CREB-H in liver of Mashen and Large White pigs at 1,30,60,90,120,150 and 180 days of age.The results showed that CREB-H gene mRNA was widely expressed in 12 tissues of sampled pigs,and the expression was high in liver and small intestine.The expression of CREB-H protein in liver was significantly higher than that in other tissues (P<0.05),and it was not expressed in heart,spleen and cerebellum.The developmental expression patterns of CREB-H gene mRNA and protein occurring in liver of pigs were affected by days of age,breed and the interaction of breed and days of age (P<0.01).The expression of CREB-H gene mRNA and protein in liver were the highest at 1 day of age after birth between two breeds,and the expression of CREB-H protein in Mashen pig was extremely significantly higher than that in Large White pig at each stage (P<0.01),and CREB-H was mainly expressed in liver of pig.There were temporal and spatial differences of the expression in liver between Mashen and Large White pigs,which might be relate to lipid metabolism in pigs at different developmental stages,this results provided a theoretical basis for studying the regulation mechanism of lipid metabolism in pigs.