玉屏风多糖对小鼠派氏结形态结构及其T细胞亚群的影响

通过研究玉屏风多糖（YPF-P）对小鼠派氏结（Peyer's patches，PPs）形态结构及其T细胞亚群的影响，探讨其对小鼠肠黏膜的免疫调节作用。选取96只SPF小鼠随机分为6组，分别为空白对照组（0.2 mL生理盐水）、YPF-P阳性对照组（YPF-P 200 mg/kg）、环磷酰胺（cyclophosvnamide，Cy）免疫抑制组（80 mg/kg Cy）及YPF-P低、中、高剂量组（100、200、400 mg/kg YPF-P），饲喂1周，取小肠PPs，常规切片HE染色后应用图像分析技术检测PPs形态结构的变化；体外培养小鼠PPs淋巴细胞，采用流式细胞术研究PPs中T淋巴细胞亚群的变化。结果表明，YPF-P对小鼠小肠PPs的生长发育具有促进作用，Cy可极显著降低小鼠小肠PPs面积、小肠纵切面面积及PPs面积和小肠纵切面面积的比值（P < 0.01），低、中、高剂量YPF-P可在一定程度上缓解Cy对小鼠小肠的损伤作用。同时，YPF-P可显著或极显著提高PPs中CD3⁺、CD4⁺ T淋巴细胞及CD4⁺/CD8⁺（P < 0.05； P < 0.01）。结果显示，YPF-P能提高Cy诱导的免疫抑制小鼠的肠黏膜免疫功能，并能促进PPs中相关T淋巴细胞增殖，对小鼠肠黏膜功能具有增强作用。

Effects of Yupingfeng Polysaccharides on Peyer's Patches Morphology and T Lymphocyte Subsets in Mice

The effects of Yupingfeng polysaccharides (YPF-P) on the morphology and T cell subsets of Peyer's patches (PPs) were studied to explore its mechanism of immune response to mice intestinal mucosa.Ninety-six SPF mice were randomly divided into 6 groups:Blank control group(0.2 mL saline),YPF-P positive control group (200 mg/kg YPF-P),immunosuppressive group (80 mg/kg cyclophosphamide) and 100 (low),200 (medium) and 400 mg/kg (high) YPF-P groups,feeding for one week.Small intestine PPs were sampled,section and HE stained.The changes of PPs morphological structure were analyzed by image analysis technology.Flow cytometry was used to study the changes in subsets of T lymphocytes isolated from murine PPs.The results showed that YPF-P promoted the growth and development of small intestine PPs in mice.Cyclophosphamide could significantly reduce the area of PPs,the cross-section area of the small intestine and their ratio(P < 0.01).YPF-P could alleviate the damage in the small intestine caused by cyclophosphamide to some extent,increase CD3⁺,CD4⁺ T lymphocytes and CD4⁺/CD8⁺ in PPs lymphocytes (P < 0.05;P < 0.01).The results showed that YPF-P could enhance the intestinal mucosal immune function of cyclophosphamide-induced immunosuppressive mice,and promote the proliferation of related T lymphocytes in PPs,which could enhance the intestinal mucosal function of mice.