| 酶法制备大米活性肽及抗氧化性的研究 |
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| 引用本文： | 申衍豪,刘芳.酶法制备大米活性肽及抗氧化性的研究[J].现代农业科技,2010(23):319-321. |
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| 作者姓名： | 申衍豪 刘芳 |
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| 作者单位： | 中南林业科技大学食品科学与工程学院; |
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| 摘 要： | 以水解液中活性多肽的得率为目标,分别采用5种蛋白酶对大米蛋白进行水解,得出最佳水解酶。水解大米蛋白然后脱盐处理,用铁氰化钾测定了大米多肽的还原能力,并考查了大米多肽对羟基自由基(.OH)、超氧阴离子(O2-.)、二苯代苦味肼酰基自由基(DPPH.)的抑制作用,并同VC做了比较。结果表明:大米蛋白制备活性肽的最佳水解工具酶为枯草杆菌碱性蛋白酶。大米多肽具有还原能力,并对这几种自由基体系具有不同的清除作用。在20.00～50.00 mg/mL时,大米多肽和VC对.OH的清除作用相当;大米多肽对O2-.的清除作用随浓度的增加而增强;在5.00 mg/mL时,大米多肽对DPPH.的清除率达到57.69%,但对O2-.和DPPH.的清除能力均低于VC。
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| 关 键 词： | 大米多肽 碱性蛋白酶 抗氧化 |
Study on the Enzymatic Preparation of Rice Antioxidant Peptides |
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| Authors: | SHEN Yan-hao LIU Fang |
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| Institution: | SHEN Yan-hao LIU Fang(College of Food Science and Engineering,Central South University of Forestry and Technology,Changsha Hunan 410004) |
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| Abstract: | Rice protein were hydrolyzed by five protease respectively,with the indexes of yield and reducing power of peptides in hydrolyzate.The results showed that the optimum enzyme was Bacillus subitilis alkali protease.The deoxidizing capability of rice peptide was evaluated by using K3(Fe(CN)6)test.The antioxidant activity of rice peptide was studied through the systems of hydroxyl radical(.OH),superoxide radical(O2-.)and DPPH.,compared with those of VC.The results show that the rice peptide had different scaven... |
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| Keywords: | rice peptides alkali protease antioxidation |
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