不同剂型苯醚甲环唑和嘧菌酯及其原药对斑马鱼的急性毒性评价

为评价不同剂型农药对水生生物的毒性影响,采用半静态试验法,测定了不同剂型苯醚甲环唑和嘧菌酯及其原药对斑马鱼的急性毒性效应。结果表明,95%苯醚甲环唑原药、3%苯醚甲环唑悬浮种衣剂、400 g·L-1苯醚甲环唑悬浮剂和60%苯醚甲环唑水分散粒剂对斑马鱼96 h的LC50值与95%置信限(括号内)分别为1.05(0.93~1.13)、1.34(1.26~1.39)、1.44(1.37~1.52)、2.72(2.68~2.76)a.i.mg·L-1;93%嘧菌酯原药、10%嘧菌酯悬浮种衣剂、25%嘧菌酯悬浮剂和50%嘧菌酯水分散粒剂对斑马鱼96 h的LC50值与95%置信限(括号内)分别为0.67(0.64~0.72)、0.88(0.85~0.92)、1.03(0.98~1.07)、1.60(1.10~1.81)a.i.mg·L-1。依据《化学农药环境安全评价试验准则》,不同剂型苯醚甲环唑及原药对斑马鱼的急性毒性级别均为中毒;25%嘧菌酯悬浮剂和50%嘧菌酯水分散粒剂对斑马鱼的急性毒性级别为中毒;93%嘧菌酯原药和10%嘧菌酯悬浮种衣剂对斑马鱼的急性毒性级别为高毒。上述结果表明,不同剂型苯醚甲环唑和嘧菌酯及其原药对斑马鱼的急性毒性存在差异,毒性从高到低依次为:原药、悬浮种衣剂、悬浮剂、水分散粒剂。

Acute Toxicity of Technical Material and Different Formulations of Difenoconazole and Azoxystrobin to Zebrafish

Different formulations of pesticides show various toxicities to organisms. In this study, an acute toxicity experiment was conducted to evaluate the ecological risks of technical material and different formulations of difenoconazole and azoxystrobin to adult zebrafish using semi-static test. The 96 h LC₅₀ values and 95% confidence limits for 95% difenoconazole technical material(TC), 3% difenoconazole flowable concentrate for seed coating(FS), 400 g·L^-1 difenoconazole aqueous suspension concentrate(SC), and 60% difenoconazole water dispersible granule(WG) for adult zebrafish were 1.05 and 0.93~1.13, 1.34 and 1.26~1.39, 1.44 and 1.37~1.52, and 2.72 and 2.68~2.76 a.i.mg·L^-1, respectively. Those of 93% azoxystrobin technical material(TC), 10% azoxystrobin flowable concentrate for seed coating(FS), 25% azoxystrobin aqueous suspension concentrate(SC), and 50% azoxystrobin water dispersible granule(WG) for adult zebrafish were respectively 0.67 and 0.64~0.72, 0.88 and 0.85~0.92, 1.03 and 0.98~1.07, and 1.60 and 1.10~1.81 a.i.mg·L^-1. According to the Experimental Guideline for Environmental Safety Evaluation of Chemical Pesticides, the toxicity of TC and different formulations of difenoconazole and that of 25% azoxystrobin SC and 50% azoxystrobin WG to adult zebrafish were moderate, while that of 93% azoxystrobin TC and 10% azoxystrobin FS was high. These results indicate that the toxicity of TC and different formulations of difenoconazole and azoxystrobin to adult zebrafish is different, being TC > FS > SC > WG.