| 肠道肠球菌AUH-HM195原生质体制备与再生条件初探 |
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| 引用本文： | 成志远,倪鑫,樊进茹,赵慧,王铭,王秀伶. 肠道肠球菌AUH-HM195原生质体制备与再生条件初探[J]. 河北农业大学学报, 2010, 33(2) |
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| 作者姓名： | 成志远 倪鑫 樊进茹 赵慧 王铭 王秀伶 |
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| 作者单位： | 河北农业大学,生命科学学院,河北,保定,071001 |
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| 基金项目： | 国家自然科学基金资助项目(30570035) |
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| 摘 要： | 应用正交试验研究菌龄、酶浓度、酶解时间和渗透压稳定剂种类对肠道肠球菌(Enterococcus hirae)AUH-HM195原生质体制备与再生的影响。结果表明:菌龄与酶浓度的交互作用对原生质体的制备和再生影响最大。最优组合为:菌龄6 h,溶菌酶浓度5 mg/mL,酶解时间1 h,渗透压稳定剂在制备时使用0.7 mol/L KCl,再生时使用17%蔗糖,制备率为93.2%,再生率为14.5%。
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| 关 键 词： | 原生质体 制备与再生 正交试验 交互作用 |
The primary exploring of the factors affecting protoplast formation and regeneration of strain Enterococcus hirae AUH-HM195 |
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| CHENG Zhi-yuan,NI Xin,FAN Jin-ru,ZHAO Hui,WANG Ming,WANG Xiu-ling. The primary exploring of the factors affecting protoplast formation and regeneration of strain Enterococcus hirae AUH-HM195[J]. Journal of Agricultural University of Hebei, 2010, 33(2) |
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| Authors: | CHENG Zhi-yuan NI Xin FAN Jin-ru ZHAO Hui WANG Ming WANG Xiu-ling |
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| Affiliation: | College of Life Sciences;Agricultural University of Hebei;Baoding 071001;China |
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| Abstract: | The effects of bacterial age,enzyme concentration,enzymolysis time and kinds of osmotic pressure stabilizer on protoplast preparation and regeneration of Enterococcus hirae AUH-HM195 were studied by orthogonal test.The results indicated that the interaction between bacterial age and enzyme concentration had obvious impact on protoplast preparation rate and regeneration rate of strain AUH-HM195.The optimal conditions were as follows: bacterial age 6 h,lysozyme concentration 5 mg/mL,enzymolysis time 1 h,osmot... |
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| Keywords: | protoplast preparation and regeneration orthogonal test interaction |
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