金佛手果叶黄酮的体外抗氧化活性研究

为了测定金佛手果叶黄酮的体外抗氧化能力,以金佛手果、叶为试材,测定果叶黄酮对O2-,·OH和DPPH的清除率.结果表明,果、叶黄酮对DPPH的清除能力IC50分别为8.25,9.03mg·L-1,稍弱于Vc和芦丁；对O2-清除能力IC50分别为17.74,20.77mg·L1,但强于芦丁,弱于Vc;对·OH清除能力IC50分别为17.06,15.86mg·L-1,清除能力强于Vc,与芦丁相差无几.方差分析显示,不同浓度果叶黄酮对O2-,·OH清除率差异极显著,且随着在反应液中浓度的增加,果叶黄酮对O2-清除率呈上升趋势,但达一定浓度时,清除率趋于平缓.果叶黄酮对·OH体系最佳作用剂量为18mg·L-1.

Antioxidative activity of flavonoids in fruit and leaf of Citrus Bergamot in vitro

Citrus Bergamot’s fruit and leaf were used as materials for determination of flavonoids’ clearance of superoxide anion free radical,hydroxyl radical and DPPH radical in order to investigate the antioxidant capacity in vitro.The results showed that the scavenging capacity of DPPH radical of fruit and leaf flavonoids’ IC50 were 8.25 and 9.03 mg·L-1 respectively,which were lower than Vc and rutin.Their superoxide anion free radical scavenge capacity IC50 were 17.74 and 20.77 mg·L-1 respectively,which were higher than rutin,but lower than Vc.Their hydroxyl radical scavenge capacity IC50 were 17.06 and 15.86 mg·L-1 respectively,which were higher than Vc,and as much as rutin.The results of variance analysis showed that there is a significant difference in scavenging rate of superoxide anion free radical and hydroxyl radical among different concentrations of fruit and leaf flavonoids.With the increasing concentration of fruit and leaf flavonoids in the reaction solution,the scavenging rate of superoxide anion free radical showed an increasing trend.But when the concentration reached a certain one,the increasing trend of scavenging rate became slower.The best dose for scavenging hydroxyl radical of fruit and leaf flavonoids was 18 mg·L-1.