基于CO II全基因的差翅亚目昆虫分子系统学研究

[目的]对基于CO Ⅱ全基因的差翅亚目昆虫的分子系统学进行研究。[方法]设计蜻蜓目昆虫CO Ⅱ全基因的非特异性引物,获得差翅亚目5科6属8种共8条CO Ⅱ全序列。采用CluxtalX1.8、ContigExpress、MEGA2.1、PHYLIP3.6a以及MrBayes V3.0等生物学软件及简约法和最大似然法对其进行分析并构建分子系统树。[结果]CO Ⅱ的A＋T含量较低（68.6%）,说明蜻蜓目是一比较原始的类群;每条序列均含有2个半胱氨酸,这一点有别于其他类群昆虫;不支持将大蜻亚科上升为科的观点;支持将大蜓科和蜓科归入蜓总科,而将春蜓科上升为总科的观点;差翅亚目5科之间的进化关系由慢到快依次为：春蜓科→大蜓科→蜓科→伪蜻科→蜻科。[结论]为蜻蜓目分子系统学研究提供相关依据。

Study on Molecular Phylogeny to the Species of Anisoptera (Insecta:Odonata) Based on CO II Complete Genes

[Objective] The research aimed to study the molecular phylogeny to the species of Anisoptera (Insecta:Odonata) based on CO II complete genes.[Method] Non-special primers were designed on the CO II genes of Odonata.CO II complete genes of 6 genera,including 8 species of 5 families in Anisoptera,had been sequenced and analyzed.The phylogenetic trees were reconstructed using CluxtalX1.8,ContigExpress,MEGA2.1,PHYLIP3.6a and MrBayes V3.0 sofewares,and maximum parsimony and maximum likelihood methods,respectively.[Result] The results show that A+T contents of CO II genes of Libellulidae are lower (68.6%) in Insecta,which proves Odonata is an original group.Every sequence includes 2 Cys,which differs from the other groups of Insecta.Do not agree with the view of raising Macrominae into Macromidae.Agree with the views of putting both Aeshnidae and Cordulegastridae into Aeshnidea,and raising Gomphidae into a superfamily.Order of the evolution relationship of 5 families is:Gomphidae→Cordulegastridae→Aeshnidae→Corduliidae→Libellulidae.[Conclusion] The study can provide the relative basis for molecular phylogeny study of Odonata.