游离棉酚对育雏期海赛克斯蛋鸡免疫功能的影响

为了研究游离棉酚对育雏期海赛克斯蛋仔鸡免疫功能的影响,本试验选用360只1日龄海赛克斯蛋雏鸡,随机分为4组,每组3个重复,每个重复30羽。Ⅰ组饲喂基础日粮,Ⅱ、Ⅲ、Ⅳ组为试验组,分别饲喂含游离棉酚70、105、140 mg/kg的日粮,进行为期42 d的饲养试验,分别在14、28、42日龄进行取样,测定免疫器官指数和血清中IL-2、IFN-γ的含量,并每周测定血清中新城疫HI抗体效价。结果显示,①日粮中不同水平的游离棉酚对雏鸡的胸腺、法氏囊、脾脏指数无明显影响;②日粮中不同水平的游离棉酚对新城疫抗体的产生无显著抑制作用(P＞0.05);③日粮中不同水平的游离棉酚能明显降低鸡血清中IL-2、IFN-γ的含量。结果表明,当游离棉酚含量高达140 mg/kg时,对雏鸡的免疫器官指数及抗体的产生没有显著影响(P＞0.05),但在第42日龄时能显著或极显著降低鸡血清中细胞因子的含量(P＜0.05或P＜0.01))。

Effects of Free Gossypol on Immunity of the HISEX Young Hens

To study the effects of free gossypol (FG) in the diets on immunity in the starter period of HISEX layers, 360 brown shell HISEX layers of 1 day of age were assigned randomly to four groups with three pens per groups and 30 chicks per pens. The chicks in control group (group Ⅰ) were fed with the basal diets and the chicks in other groups were fed the diets containing free gossypol, 70,105 and 140 mg/kg, respectively. The trial lasted for 6 weeks from 1 to 42 days old. On 14, 28 and 42 days, indices of immune organs and the level of cytokines in the sera were measured. Sera HI of Newcastle disease was detected once a week. The results showed that the organ index of bursa of fabricius, thymus and spleen were not influenced by the level of FG in the diets.Different levels of free gossypol had no significant effect on ND antibody titre of HISEX layers. Different levels of free gossypol decrease levels of sera cytokine IL-2,IL-4 and IFN-γ. It was concluded that up to 140 mg/kg of FG had no significant effect on immune organ index and titer of Newcastale disease. But it could decrease level of serum cytokine on 42 days.