抗菌肽[L6,K11]-IsCT抑菌性能及其稳定性研究

IsCT最初是从蝎子中分离出的一种非细胞选择性线性抗菌肽,为了将其应用于动物饲料,需研究其对常见致病菌的抑制和杀灭作用。本试验采用微量稀释法和琼脂扩散法对ISCT衍生物L⁶,K¹¹]-IsCT的抗菌活性及其稳定性进行探索。结果显示,相对于金黄色葡萄球菌,L⁶,K¹¹]-IsCT对大肠杆菌具有更好的抑制作用,其对大肠杆菌的最小抑菌浓度为50 μg/mL。另外,该衍生物对高温表现出很好的稳定性,同时具有很好的酸碱耐受性和人工胃液耐受性。经121 ℃、0.12 MPa处理30 min,以及2.0≤pH≤11.0处理30 min或人工胃液2 h孵育处理后,依然能保持很好的抑菌活性。

Antibacterial Activity and Stability Study of Antimicrobial Peptides [L6, K11]-IsCT

IsCT was a non-cell-selective antimicrobial peptide isolated from the scorpion.In order to be used in animal feed,HT5] HT5"]it was necessary to study the effects on inhibiting and killing common pathogenic bacteria. Agar gel diffusion and dilution methods were used in this experiment to study the antibacterial activities and stabilities of L⁶,K¹¹]-IsCT which was the derivative of IsCT. The results indicated that L⁶,K¹¹]-IsCT had better antibacterial activities to Escherichia coli than Staphylococcus aureus. The minimal inhibitory concentration (MIC) of Escherichia coli was 50 μg/mL. When treated with 121 ℃ 0.12 MPa for 30 minutes or 2.0≤pH≤11.0 for 30 minutes and incubated in artificial gastric juice for 2 h,L⁶,K¹¹]-IsCT remained excellent antibacterial activities. Therefore,L⁶,K¹¹]-IsCT presented excellent heat stablility and could resist to extreme pH environment as well as artificial gastric juice.