构树叶提取物对金黄色葡萄球菌的抑制作用及机理研究

为了研究构树叶提取物的抑菌作用及其机理,以金黄色葡萄球菌为供试菌,采用牛津杯法测定构树叶提取物的抑菌效果,通过测定供试菌生长曲线、细胞膜渗透性和蛋白质合成来研究构树叶提取物的抑菌机理。结果表明,构树叶水提取物和75%乙醇提取物抑菌圈直径分别为17.5和18.0 mm,最小抑菌浓度均为6.8 mg/mL,最小杀菌浓度均为12.50 mg/mL。构树提取物能显著抑制金黄色葡萄球菌蛋白质合成,从而抑制其生长,但对其细胞膜的通透性影响不大。因此,构树叶提取物通过抑制金黄色葡萄球菌蛋白质合成发挥其抑菌效果。

Study on Inhibition Effect of Broussonetial papyrifera Leaf Extract on Staphylococcus aureus and its Mechanism

To investigate the inhibition effect of Broussonetia papyrifera leaf extract and its mechanism, the Staphylococcus aureus was used as test bacteria, the inhibition zone diameters were observed by the Oxford cup method, the growth curve, protein synthesis, and the membrane permeability of the the Staphylococcus aureus were detected to study the antibacterial mechanism of the Broussonetial papyrifera leaf extract. The results indicated that the antibacterial circle diameter of the Broussonetial papyrifera leaf extract and 75% ethanol extract were 17.5 and 18.0 mm respectively, the minimal inhibitory concentration (MIC) was 6.8 mg/mL, and the minimum bactericidal concentration (MBC) was 12.5 mg/mL. The results also revealed that the growth of the Staphylococcus aureus significantly decreased owing to the inhibition of the bacterial protein synthesis caused by the Broussonetial papyrifera leaf extract, but the membrane permeability was not affected. The Broussonetial papyrifera leaf extract showed obvious antibacterial activity on Staphylococcus aureus in vitro, and the antibacterial effect was probably associated with protein synthesis inhibition of the bacteria.