荷斯坦奶牛子宫内膜上皮细胞的体外培养

为建立和完善奶牛子宫内膜上皮细胞的体外培养技术,采用组织培养和胶原酶消化相结合的方法,对中国荷斯坦奶牛的子宫内膜上皮细胞进行了原代分离培养,经胰酶差时消化进行纯化,并以免疫组化法检测角蛋白表达。结果显示,所采用的方法可高效获得呈铺路石样的上皮细胞,角蛋白表达呈阳性,第2~8代的传代细胞在形态特征和增殖状态上保持着与原代细胞相近的生物学特征。

Cell Culture of Endometrial Epithelial Cells from Chinese Holstein Dairy Cows in vitro

To establish and perfect cell culture technique of endometrial epithelial cell (EnEpC)in dairy cows, primary cell was isolated and cultured by tissue cultivation that combined with enzymolysis of collagenase. The endometrial epithelial cell was purified from primary culture cells by different time digestion of trypsase, and EnEpC was identified by immunohistochemical detection of cytokeratin expression. The results demonstrated that flagstone-like epithelial cell was obtained by the adoptive method, and cytokeratin expression was positive in the cells. The purified cells could be subcultured about 10 times, and cells from generation 2 to 8 maintained stably biological characteristics of morphology and proliferation state that were found in primary culture cells.