Effects of the E1 activating enzyme UBA2 on porcine oocyte maturation,apoptosis, and embryonic development in vitro |
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| Authors: | Da Xu Jing Bi Yunfeng Guan Xiaotong Luo Xuan Chen Yanqiu Lv Yi Jin |
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| Institution: | 1. Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China;2. College of Life Sciences, Xiamen University, Fujian, China |
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| Abstract: | The purpose of this study was to investigate the effect of the E1 activating enzyme UBA2 on the expression of the SUMO-1 protein during in vitro maturation (IVM) of pig oocytes and embryonic development. In the 5 μg/ml UBA2 treatment group, the expression of the anti-apoptotic gene Bcl-2 and the embryo cleavage rate was significantly increased, while the proapoptotic gene Bax was significantly reduced. When 10 μg/ml UBA2 was added, the in vitro maturation rate, blastocyst rate, and SUMO-1 protein content of oocytes increased significantly (p < .05), and the expression of proapoptotic gene Caspase3 was significantly decreased (p < .05), while the viability of cumulus cells was extremely significantly reduced (p < .01). In summary, UBA2 can regulate the content of the SUMO-1 protein in mature pig oocytes in vitro, which in turn affects the maturation rate of oocytes, expression of apoptosis genes, cumulus cell viability, and the development of embryos after fertilization. |
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| Keywords: | embryo in vitro porcine oocyte SUMO-1 UBA2 |
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