| 利用EDTA分析大麦发芽过程中蛋白质的溶解 |
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| 引用本文： | 韩丹,莫新迎,王晓丹,李明达,赵长新.利用EDTA分析大麦发芽过程中蛋白质的溶解[J].种子,2010,29(6). |
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| 作者姓名： | 韩丹 莫新迎 王晓丹 李明达 赵长新 |
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| 作者单位： | 大连工业大学生物与食品工程学院,辽宁,大连,116034 |
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| 基金项目： | 国家科技部"十一五"科技支撑项目 |
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| 摘 要： | EDTA是一种良好的金属离子螯合剂。本试验分别用不同浓度的EDTA水溶液培养大麦,并与水培养做对比研究。结果表明,EDTA可以通过螯合维持蛋白酶活力所必须的金属离子降低麦芽中蛋白酶活力,并改变麦芽中各蛋白含量。运用SDS-PAGE电泳进一步分析麦芽蛋白,从其组分的变化上分析出贮藏蛋白向可溶性蛋白转化的具体方式。
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| 关 键 词： | 大麦蛋白溶解度 蛋白酶活力 蛋白含量 SDS-PAGE电泳 |
Using EDTA to Analyze Protein Dissolvation of Barley During Germination |
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| HAN Dan,MO Xin-ying,WANG Xiao-dan,LI Ming-da,ZHAO Chang-xin.Using EDTA to Analyze Protein Dissolvation of Barley During Germination[J].Seed,2010,29(6). |
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| Authors: | HAN Dan MO Xin-ying WANG Xiao-dan LI Ming-da ZHAO Chang-xin |
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| Institution: | HAN Dan,MO Xin-ying,WANG Xiao-dan,LI Ming-da,ZHAO Chang-xin(Dalian Polytechnic University College malting buckwheat of Biology and Food Technology,Dalian 116034,China) |
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| Abstract: | EDTA is a kind of good metal ions chelating agent.Different concentrations EDTA aqueous solution were used to culture barley,and compared with ones cultured by water.The results showed that EDTA could reduce the protease activity by chelating metal ions which are required to hold the protease activity,and change the content of each protein in malt.Using SDS-PAGE to analyze malt protein further could find concrete way of the transform from reserve protein to soluble protein in the change of protein component... |
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| Keywords: | EDTA |
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