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Variation in phenotype and proteins in plants regenerated from cell suspensions of potato cv. BP1
Authors:J. M. Lindeque  A. van der Mescht  M. M. Slabbert  G. Henn
Affiliation:(1) Vegetable and Ornamental Plant Research Institute, Private Bag X293, Pretoria, RSA;(2) Plant Biotechnology Research Centre, Private Bag X293, Pretoria, RSA
Abstract:Summary Phenotypic variation, SDS-PAGE and protein-DNA binding were used to determine variation during the in vitro phase of potato plantlets derived from callus and cell suspensions. Of the 27 plantlets assessed. 3 displayed a low or abnormal growth, 16 normal growth which correlated well with the original explant and 9 showed strong or vigorous growth. Differences were not observed in the polypeptide profiles of these plantlets. However distinct differences in the protein-DNA-binding profiles occurred which correlated well with the phenotypic variation observed.
Keywords:Potato  protein-DNA-binding  SDS PAGE  Solanum tuberosum  somaclonal variation
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