| 乳铁蛋白的分离纯化 |
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| 引用本文： | 曲练达,王术德,侯喜林,朴范泽. 乳铁蛋白的分离纯化[J]. 黑龙江八一农垦大学学报, 2006, 18(3): 61-63 |
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| 作者姓名： | 曲练达 王术德 侯喜林 朴范泽 |
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| 作者单位： | 黑龙江八一农垦大学动物科技学院, 大庆 163319;东北农业大学 |
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| 摘 要： | 乳铁蛋白的分离纯化方法较多,本文采用3次盐析法和1次凝胶层析法从来源丰富的牛初乳中分离纯化LF并利用电泳和分光光度法法检测其纯度和含量,所获得的乳铁蛋白的分子质量为90000u,纯度大约为92%。
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| 关 键 词： | 乳铁蛋白 盐析 凝胶层析 分光光度法 |
| 文章编号： | 1002-2090（2006）03-0061-03 |
| 收稿时间： | 2006-04-22 |
| 修稿时间： | 2006-04-22 |
Isolation and Purification of Lactoferrin |
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| QU Lian-da, WANG Shu-de, HOU Xi-lin et al.. Isolation and Purification of Lactoferrin[J]. journal of heilongjiang bayi agricultural university, 2006, 18(3): 61-63 |
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| Authors: | QU Lian-da WANG Shu-de HOU Xi-lin et al. |
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| Abstract: | There were lots of methods to isolate and purify LF, in this experiment, 3 times salt fractionation and 1 time chromatographic were adopted to isolate and purify LF from colostral milk. Electrophoresis and absorption spectrometry were also utilized to detect its fineness and contents. The molecular mass of LF was 90 000 u and the fineness was 92%. |
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| Keywords: | lactoferrein salting-out gel chromatography absorption spectrometry |
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