| 胰岛素、胰高血糖素对体外培养肝细胞DGAT2mRNA的影响 |
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| 引用本文： | 王娅,邓俊良,左之才,任志华,胡延春. 胰岛素、胰高血糖素对体外培养肝细胞DGAT2mRNA的影响[J]. 兽医大学学报, 2012, 0(11): 1716-1719 |
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| 作者姓名： | 王娅 邓俊良 左之才 任志华 胡延春 |
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| 作者单位： | 四川农业大学动物医学院“动物疫病与人类健康”和“环境公害与动物疾病”四川省重点实验室,四川雅安625014 |
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| 基金项目： | 教育部《长江学者和创新团队发展计划》创新团队项目（IRT0848）;四川省教育厅重点项目（10ZA048）;四川农业大学优秀学士毕业论文培育计划项目 |
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| 摘 要： | 为阐明神经内分泌因子胰岛素、胰高血糖素在奶牛脂肪代谢过程中的调控作用，用荧光定量PCR方法检测胰岛素、胰高血糖素对肝细胞二酰基甘油酰基转移酶（DGAT2）mRNA丰度的影响。结果表明低浓度的胰岛素能促进脂肝细胞内DGAT2mRNA的表达；胰高血糖素抑制脂肝细胞内DGAT2mRNA表达。由此证明：胰岛素和胰高血糖素能直接调控肝细胞中的DGAT2基因mRNA的表达。
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| 关 键 词： | 胰岛素 胰高血糖素 肝细胞 DGAT2 mRNA |
Effects of INS and GLP on abundance of DGAT2 mRNA in vitro culture bovine hepatocyte cells |
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| WANG Ya,DENG Jun-liang,ZUO Zhi-cai,REN Zhi-hua,HU Yan-chun. Effects of INS and GLP on abundance of DGAT2 mRNA in vitro culture bovine hepatocyte cells[J]. , 2012, 0(11): 1716-1719 |
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| Authors: | WANG Ya DENG Jun-liang ZUO Zhi-cai REN Zhi-hua HU Yan-chun |
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| Affiliation: | (College of Veterinary Medicine, Sichuan Agricultural University, Key Laboratory of Animal Disease and Human Health, Key Laboratory of Environmental Hazard and Animal Diseases, Ya ＇ an, Sichuan 625014, China) |
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| Abstract: | In order to investigate the regulation roles of INS and GLP in the lipometabolism of dairy cows,expression of DGAT2 mRNA of cultured neonatal calf hepatocyte was abserved by fluores- cent quantitative RT-PCR ; the expression of DGAT2 mRNA increased with increasing concentra- tions of INS,and inhibited with increasing concentrations of GLP in the media. These results indi- cate INS and GLP could regulate the expressions of cultured hepatocyte cells DGAT 2 mRNA directly. |
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| Keywords: | INS GLP hepatocyte DGAT2 mRNA |
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