紫花地丁黄酮类提取物体外抗传染性支气管炎病毒作用研究 |
| |
引用本文: | 伍小波,古淑英,罗先钦,罗忠亮.紫花地丁黄酮类提取物体外抗传染性支气管炎病毒作用研究[J].兽医大学学报,2012(11):1694-1697. |
| |
作者姓名: | 伍小波 古淑英 罗先钦 罗忠亮 |
| |
作者单位: | [1]西南大学药学院,重庆400715 [2]重庆市中药研究院,重庆400065 |
| |
基金项目: | 西南大学青年基金资助项目(SWU209011);重庆市科技创新能力建设基金资助项目(2009CB1010) |
| |
摘 要: | 为了在体外研究紫花地丁黄酮类提取物抗鸡传染性支气管炎病毒(IBV—M41)的作用,以鸡胚肾细胞为宿主细胞,阿昔洛韦为阳性对照药物,通过观察细胞病变效应(CPE)与空斑减数试验测定紫花地丁黄酮类提取物抗IBV活性,计算其IC50与治疗指数,并从药物对病毒的直接灭活作用、对病毒吸附的影响及对病毒穿膜的影响3个方面初探紫花地丁黄酮类提取物抗IBV活性的机理。结果:紫花地丁黄酮类提取物能明显抑制IBV的致病变作用,其IC50为16.52mg/L,TI值为15.76。研究显示,紫花地丁黄酮类提取物在体外对IBV直接灭活的效果明显,在高浓度时对抑制IBV吸附与穿入细胞具有一定作用。结果表明:紫花地丁黄酮类提取物在体外有明显的抗IBV感染作用,且主要是通过直接灭活IBV而发挥作用。
|
关 键 词: | 紫花地丁 黄酮 鸡传染性支气管炎病毒 细胞病变效应法(CPE) 空斑减数试验 |
The in vitro antiviral activity of the flavonoids extract from the Chinese medicinal herb Viola yedoensis |
| |
Authors: | WU Xiao-bo GU Shu-ying LUO Xian-qin LUO Zhong-liang |
| |
Institution: | 1. College of Pharmaceutical Sci- ences ,Southwest University, Chongqing 400715, China ; 2. Chongqing Academy of Chinese Mate- ria Medica , Chongqing 400065, China) |
| |
Abstract: | The antiviral activity of an extract from the Chinese medicinal herb Viola yedoensis has been investigated in this experiment. The flavonoids extract was tested for its antiviral activity a- gainst infectious bronchitis virus (IBV) via cytopathic effect reduction assay and plaque reduction assay in cell culture. The initial studies on antiviral mechanism consisted of three aspects: virucidal assay,adsorption assay and penetration assay, respectively. The flavonoids extract presented a sig- nificant antiviral activity against IBV and had a low cytotoxicity towards chicken embryo kidney (CEK) cells. The 50% inhibitory concentration (IC50) was 16.52 mg/L (TI=15. 76). At a high concentration of the flavonoids extract (100 mg/L) ,the quantity of plague of IBV was reduced ap- proximately by 90%. The flavonoids extract was able to inactivate virus directly. It is concluded that the flavonoids extract from the Chinese medicinal herb Viola yedoensis has an anti-IBV activi- ty by inactivating virus directly. |
| |
Keywords: | Viola yedoensis flavonoids infectious bronchitis virus CPE assay plaque reduction assay |
本文献已被 维普 等数据库收录! |