| 应用正交设计对组培杜梨快速繁殖的研究 |
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| 引用本文： | 李丹丹,王忆,韩振海,许雪峰,孔瑾,李天忠.应用正交设计对组培杜梨快速繁殖的研究[J].北方园艺,2009(1). |
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| 作者姓名： | 李丹丹 王忆 韩振海 许雪峰 孔瑾 李天忠 |
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| 作者单位： | 中国农业大学园艺植物研究所,北京市果树逆境生理与分子生物学重点实验室,北京,100193;中国农业大学园艺植物研究所,北京市果树逆境生理与分子生物学重点实验室,北京,100193;中国农业大学园艺植物研究所,北京市果树逆境生理与分子生物学重点实验室,北京,100193;中国农业大学园艺植物研究所,北京市果树逆境生理与分子生物学重点实验室,北京,100193;中国农业大学园艺植物研究所,北京市果树逆境生理与分子生物学重点实验室,北京,100193;中国农业大学园艺植物研究所,北京市果树逆境生理与分子生物学重点实验室,北京,100193 |
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| 摘 要： | 以杜梨试管苗为材料,应用正交试验法研究不同植物激素及其配比对试管苗生长与增殖生长的影响.结果表明:适合杜梨试管苗生长阶段的培养基为MS培养基+6-BA 2.0mg/L+IBA 0.1 mg/L+GA3 0.1 mg/L;适合杜梨增殖生长的培养基为MS培养基+6-BA 1.0mg/L+IBA 0.6mg/L+GA3 0.6mg/L.
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| 关 键 词： | 杜梨 组织培养 激素 |
Studies on Rapid Propagation Tissue Culture in Pyrus betulifolia Bunge by Applying Orthogonal Design |
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| LI Dan-dan,WANG Yi,HAN Zhen-hai,XU Xue-feng,KONG Jin,LI Tian-zhong.Studies on Rapid Propagation Tissue Culture in Pyrus betulifolia Bunge by Applying Orthogonal Design[J].Northern Horticulture,2009(1). |
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| Authors: | LI Dan-dan WANG Yi HAN Zhen-hai XU Xue-feng KONG Jin LI Tian-zhong |
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| Institution: | Institute for Horticulture Plants;China Agriculture University;Key Laboratory of Beijing Municipality of Stress Physiology and Molecular Biology for Fruit Tree;Beijing 100193;China |
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| Abstract: | Using orthogonal design methods,the effects of different hormone concentration and rate on the rapid propagation of Pyrus betulaefolia Bunge.were studied.The results showed that the best culture medium for the growth of Pyrus betulaefolia was MS with BA 2.0 mg/L + IBA 0.1 mg/L +GA30.1 mg/L.The results also indicated that MS supplied with BA 1.0 mg/L + IBA 0.6 mg/L + GA30.1 mg/L was good for the propagation of Pyrus betulaefolia. |
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| Keywords: | Pyrus betulaefolia Bunge Tissue culture Hormone |
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