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Identification of Arcanobacterium pluranimalium by matrix-assisted laser desorption ionization-time of flight mass spectrometry and,as novel target,by sequencing pluranimaliumlysin encoding gene pla |
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| Authors: | A. Balbutskaya O. Sammra S. Nagib M. Hijazin J. Alber C. Lämmler G. Foster M. Erhard P.N. Wragg A. Abdulmawjood E. Prenger-Berninghoff |
| Affiliation: | 1. Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstr 81, 35392 Gießen, Germany;2. SAC Consulting Veterinary Services, Drummondhill, Stratherrick Road, Inverness IV2 4JZ, UK;3. RIPAC-LABOR GmbH, Am Mühlenberg 11, 14476 Potsdam-Golm, Germany;4. Animal Health and Veterinary Laboratories Agency, Penrith Regional Laboratory, Merrythought, Calthwaite, Penrith CA11 9RR, UK;5. Institut für Lebensmittelqualität und-sicherheit, Stiftung Tierärztliche Hochschule Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany;6. Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität, Frankfurterstr. 85-91, 35392 Gießen, Germany |
| Abstract: | In the present study 13 Arcanobacterium pluranimalium strains isolated from various animal origin could successfully be identified phenotypically by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and genotypically by sequencing 16S rDNA and the pluranimaliumlysin encoding gene pla. The detection of mass spectra by MALDI-TOF MS and the novel genotypic approach using gene pla might help to identify A. pluranimalium in future and might elucidate the role this species plays in infections of animals. |
| Keywords: | MALDI-TOF MS 16S rDNA Pluranimaliumlysin |
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