用幼穗组织培养筛选小麦耐盐突变体 |
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引用本文: | 肖海林,赵世绪. 用幼穗组织培养筛选小麦耐盐突变体[J]. 核农学报, 1989, 3(2): 85-90 |
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作者姓名: | 肖海林 赵世绪 |
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作者单位: | 北京农业大学生物学院,北京农业大学生物学院 北京 ,北京 |
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摘 要: | 运用组织培养研究了小麦耐盐(NaCl)突变体的筛选,获得了大量耐盐细胞系,其中有5个系的耐盐性稳定而且较强,其余的系在以后继代培养中耐盐性逐步消失。耐盐稳定的细胞系在含NaCl的培养基中再生了植株,其中三棵植株表现了耐盐特性,而且两株的耐盐性通过有性世代仍保留,初步确定为遗传的耐盐特性突变体。在试验中采用了60Co γ射线辐照幼穗,结果表明1kR比较有效。
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关 键 词: | 组织培养 NaCl耐性突变体 小麦 |
收稿时间: | 2009-12-31 |
修稿时间: | 2009-12-31 |
THE SELECTION OF NaCI-TOLERANT MUTANTS BY TISSUE CULTURE IN WHEAT |
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Abstract: | The selection of NaCl-tolerant mutants in wheat was studied,A number of NaCl-tolerant cell lines were selected from a great quantity ofcallus cultures.The NaCl-tolerance of 5 lines were stable and very strongafter subculture of three times. The plants were regenerated on 1.0%NaCl-containing media.The NaCl-tolerance appeared in the callus from embryos of three plants regenerated.Two of the three plants which could pass on the NaCl-tolerance to next generation through gametophyte are preliminarily considered as NaCl-tolerant genetic mutant. 60Co γ-ray was used to induce the mutation and 1kR was found to be effective. |
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Keywords: | tissue culture NaCl-tolerance mutant wheat |
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