百香果茎基腐病菌分离鉴定与药剂筛选研究

为研究百香果产业中多发病害茎基腐病的病原菌鉴定与防治问题,在广东省百香果主产区采集茎基腐病病株,结合形态学观察与分子测序方法鉴定分离病原菌菌株,并对其进行致病性检测,同时检测系列杀菌药剂对病菌生长及其致病性的影响。结果表明,通过形态特征观察、分子生物学鉴定,从百香果茎基腐病病株中分离获得的8个菌株鉴定为腐皮镰刀菌(Fusarium solani)。离体和活体致病力测试结果显示,致病力较高的菌株为FP6、FP7、FP2,其次为FP1、FP3、FP8、FP4,最低为FP5。不同药剂处理对病菌的生长及致病力均产生影响,其中30%苯甲嘧菌酯处理的抑制效果为最佳。研究结果可为后续百香果茎基腐病病理学、百香果抗病育种等研究提供基础。

Isolation and Identification of the Pathogen Causing Stem Rot Disease in Passiflora edulia and Fungicide Screening

The paper aims to find out the pathogen which causes stem rot disease in passion fruit, and the chemicals to control this disease effectively. Stem rot diseased samples were collected from different Passiflora edulia production fields in Guangdong. The pathogen was isolated and determined by methods of morphological observation and molecular identification. The strains’ pathogenicity was measured, and their sensibility to eight fungicides was also detected. Results showed that eight isolated pathogen strains were identified as Fusarium solani. Based on in vivo and in vitro tests with Passiflora edulis, strains of FP6, FP7 and FP2 showed highest pathogenicity, followed by FP1, FP3, FP8 and FP4, and the lowest was FP5. Different fungicides showed effects on pathogen growth rate and pathogenicity, and 30% benazoxystrobin showed the best inhibition effects. This study could lay a material and technology base for further research on pathogenic mechanism of stem rot disease, and disease resistance breeding program of passion fruit.