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青海高原油菜蜜稳定同位素产地特征与环境响应机制研究
引用本文:谭阳,刘志,韩燕,崔明明,肖明,耿贵工,孙小凤,袁玉伟. 青海高原油菜蜜稳定同位素产地特征与环境响应机制研究[J]. 核农学报, 2021, 35(3): 635-642. DOI: 10.11869/j.issn.100-8551.2021.03.0635
作者姓名:谭阳  刘志  韩燕  崔明明  肖明  耿贵工  孙小凤  袁玉伟
作者单位:青海大学农林科学院/农业农村部农产品质量安全风险评估实验室,青海西宁810016;浙江省农业科学院农产品质量标准研究所/农业农村部农产品信息溯源重点实验室,浙江杭州310021;浙江省农业科学院农产品质量标准研究所/农业农村部农产品信息溯源重点实验室,浙江杭州310021;青海大学农林科学院/农业农村部农产品质量安全风险评估实验室,青海西宁810016
基金项目:国家农产品质量安全风险评估重大专项(GJFP201701004)
摘    要:为探究青海油菜蜜稳定同位素特征及影响因素,本研究利用元素分析-稳定同位素比率质谱(EA-IRMS)建立了油菜蜜总δ13C值及其内源蛋白质δ13C、δ15N、δ2H、δ18O值的检测方法,分析了青海不同产地、不同年份油菜蜜及其内源蛋白质同位素特征差异,探讨了油菜蜜内源蛋白质稳定同位素与产地环境之间的关系.结果表明,青海省...

关 键 词:油菜蜜  内源蛋白质  稳定同位素分析  产地环境因子  分馏响应机制
收稿时间:2019-09-23

Stable Isotope Signatures of Rape Honey in Qinghai Province and Their Response Mechanisms to Environmental Factors
TAN Yang,LIU Zhi,HAN Yan,CUI Mingming,XIAO Ming,GENG Guigong,SUN Xiaofeng,YUAN Yuwei. Stable Isotope Signatures of Rape Honey in Qinghai Province and Their Response Mechanisms to Environmental Factors[J]. Acta Agriculturae Nucleatae Sinica, 2021, 35(3): 635-642. DOI: 10.11869/j.issn.100-8551.2021.03.0635
Authors:TAN Yang  LIU Zhi  HAN Yan  CUI Mingming  XIAO Ming  GENG Guigong  SUN Xiaofeng  YUAN Yuwei
Affiliation:1Academy of Agriculture and Forestry Sciences, Qinghai University/Laboratory of Quality & Safety Risk Assessment for Agro-products, Ministry of Agriculture and Rural Affairs, Xining, Qinghai 810016; 2Institute of Quality and Standard for Agro-product, Zhejiang Academy of Agricultural Sciences/Key Laboratory of Information Traceability of Agricultural Products, Ministry of Agriculture and Rural Affairs, Hangzhou, Zhejiang 310021
Abstract:In order to explore the characteristics and influencing factors on stable isotopes of Qinghai rape honey, a detection method using elemental analysis-stability isotope ratio mass spectrometry (EA-IRMS) was established to obtain the total δ13C value of rape honey and its δ13C, δ15N, δ2H, δ18O values of endogenous proteins. The stable isotope variations of rape honey and its endogenous proteins were analyzed across different years and producing areas in Qinghai. The relationship between stable isotope of endogenous protein in rape honey endogenous protein and the origin environment was also discussed. The results showed that the δ13C and δ15N values of endogenous proteins in Qinghai rape honey were considerably stable, and there were no significant differences among different years and different origins. The δ18O values of inter-annual in endogenous proteins of rape honey samples were significantly different, as well as δ18O and δ2H values of endogenous proteins from different regions. For the environment factors, δ18O value in endogenous proteins of rape honey was negatively correlated with precipitation during the production season (r=-0.822). The results of this study provided theoretical basis and technical support for the authenticity identification and origin traceability of Qinghai rape honey.
Keywords:rape honey  endogenous protein  stable isotope analysis  environmental factor  fractionation mechanism  
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