半边红李炭疽病病害调查及病原菌鉴定

探明绥江半边红李炭疽病为害程度及病原菌的种类,可为李树炭疽病的防控提供依据。以绥江县2个主要种植区会仪镇和新滩镇的6个半边红李果园为研究对象,对炭疽病为害状况进行了调查,并对炭疽病原菌进行分离保存;利用病原菌分生孢子形态及核糖体ITS序列对绥江半边红李树炭疽病致病菌进行了种类鉴定和分析。病害调查表明,新滩镇比会仪镇的危害程度严重。单孢分离获得15株分离株,各分离株平板菌落形态略有差异,获得4类不同的分生孢子,经回接验证均致病。rDNA ITS序列系统进化分析初步推定半边红李炭疽病的病原菌为胶孢炭疽复合群(Colletotrichum gloeosporioides species complex)和暹罗炭疽复合群C. siamense species complex。该研究探查了半边红李炭疽病的为害状况,获得了李炭疽菌的单胞分离菌株,初步鉴定了半边红李炭疽菌归属的复合群。

Anthracnose Disease on Prunus salicina ‘Banbianhong’: Investigation and Pathogen Identification

The aim is to investigate the anthracnose disease and the pathogen species in Prunus salicina ‘Banbianhong’ and provide a basis to prevent and control the disease. In this study, six P. salicina ‘Banbianhong’ orchards were taken as research objects in two major production areas, Huiyi and Xintan Town in Suijiang County, Yunnan Province, anthracnose disease was investigated and pathogenic fungi were isolated and stored. The pathogen species were identified and analyzed according to the spore morphology and ITS sequences of Colletotrichum. Disease investigation showed that the disease severity of anthracnose in Xintan was higher than that in Huiyi. A total of 15 single spore isolates were obtained, and the morphology of colonies on every strain plate had weak difference. Four kinds of conidia were obtained and all of them were pathogenic by Tie-back experiment. rDNA ITS sequence phylogenetic analysis showed that the pathogen of anthracnose disease in P. salicina ‘Banbianhong’ were Colletotrichum gloeosporioides species complex and C. siamense species complex. This study investigated the damage status of anthracnose disease in P. salicina ‘Banbianhong’, got the single spore isolates, and determined the complex groups of the pathogen of anthracnose disease in P. salicina ‘Banbianhong’.