| 燕麦属植物核糖体DNA染色体定位及45S rDNA的系统进化分析 |
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| 引用本文： | 王炳策,刘晓娟,程斌,任明见,徐如宏,张素勤,张立异,何方. 燕麦属植物核糖体DNA染色体定位及45S rDNA的系统进化分析[J]. 作物杂志, 2021, 37(4): 10-8. DOI: 10.16035/j.issn.1001-7283.2021.04.002 |
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| 作者姓名： | 王炳策 刘晓娟 程斌 任明见 徐如宏 张素勤 张立异 何方 |
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| 作者单位： | 1贵州大学农学院/国家小麦改良中心贵州分中心,550025,贵州贵阳2贵州省农业科学院旱粮研究所,550006,贵州贵阳 |
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| 基金项目： | 国家自然科学基金地区项目“利用Seq-BSA技术准确定位小麦新抗源贵协3号的抗条锈病基因”(31660393);贵州省基金重点项目“小麦抗源贵协3号抗条锈病基因YrGX3的精细定位”(黔科合基础[2019]450号);贵州省农业科学院青年科技基金“小麦贵协3号抗条锈病基因的细胞学定位分析”(黔农科院青年基金[2018]02号) |
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| 摘 要： | 由于缺乏明确的二倍体供体信息,燕麦属植物的起源和系统进化关系一直存在争议。利用荧光原位杂交（fluorescence in situ hybridization,FISH）方法,检测45S rDNA和5S rDNA在燕麦属不同倍性植物染色体上的位点信息;并依据已公开的45S rDNA ITS区全长DNA序列构建分子进化树。探讨燕麦属植物在不同基因组中45S rDNA的位点变化、进化规律以及分化机制,为探究燕麦属物种的起源与演化提供参考。
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| 关 键 词： | 燕麦属 45SrDNA 荧光原位杂交 ITS |
| 收稿时间： | 2020-08-05 |
Chromosomal Localization of Ribosomal DNA and Phylogenetic Analysis of 45S rDNA in Avena |
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| Wang Bingce,Liu Xiaojuan,Cheng Bin,Ren Mingjian,Xu Ruhong,Zhang Suqin,Zhang Liyi,He Fang. Chromosomal Localization of Ribosomal DNA and Phylogenetic Analysis of 45S rDNA in Avena[J]. Crops, 2021, 37(4): 10-8. DOI: 10.16035/j.issn.1001-7283.2021.04.002 |
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| Authors: | Wang Bingce Liu Xiaojuan Cheng Bin Ren Mingjian Xu Ruhong Zhang Suqin Zhang Liyi He Fang |
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| Affiliation: | 1College of Agriculture, Guizhou University/Guizhou Subcenter of National Wheat Improvement Center, Guiyang 550025, Guizhou, China2Institute of Upland Crops, Guizhou Academy of Agriculture Sciences, Guiyang 550006, Guizhou, China |
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| Abstract: | Due to the incomplete information on the diploid donor, the origin and phylogenetic relationship of oats have been controversial. The fluorescence in situ hybridization (FISH) method was used to detect the location information of 45S rDNA and 5S rDNA on the chromosomes of different ploidy plants of Avena. The molecular evolution tree was constructed based on the published full-length DNA sequence of the 45S rDNA ITS region. This paper discussed the locus changes, evolution laws, and differentiation mechanisms of 45S rDNA in different genomes of oats plants and provided references for exploring the origin and evolution of oat species. |
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| Keywords: | Avena L. 45S rDNA Fluorescence in situ hybridization ITS |
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