60Co-γ射线辐射对4种百合的诱变效应

为了探究⁶⁰Co-γ射线辐射对百合的诱变效应,以卷丹百合、金百合、湖北百合、岷江百合鳞茎为试验材料,采用不同剂量(0、2、4、6 Gy)⁶⁰Co-γ射线进行辐射处理,统计分析其辐射后的出苗率、性状表现、生理生化指标及变异状况。结果表明,随着辐射剂量的增加,金百合、湖北百合、岷江百合的出苗率、株高、开花率、产量均较对照降低。卷丹百合在辐射剂量为2 Gy时与对照差异不显著;而辐射剂量为4 Gy时,4个百合品种出苗率与对照相比差异显著;辐射剂量达到6 Gy时,鳞茎均未出苗。2 Gy辐射处理对4个百合品种植株的叶绿素含量影响较小,4 Gy处理时叶绿素含量则下降;所有品种超氧物歧化酶(SOD)活性均先升高后降低,丙二醛(MDA)含量均逐渐增强。随着辐射剂量的增加,卷丹百合的可溶性糖含量先升高后降低,其他品种的可溶性糖含量逐渐增加。金百合、湖北百合、岷江百合适宜辐射剂量分别为2~4 Gy、2 Gy、2 Gy,而卷丹百合在本试验中未找到适宜辐射剂量。本研究为百合的辐射育种提供了一定的技术参考。

Mutagenic Effects of 60Co-γ Irradiation on Four Species of Lilium

To investigate the mutagenic effects of different radiation doses of ⁶⁰Co-γ rays on Lilium, the seedballs of Lilium lancifolium Thunb, Lilium bosniacum, Lilium henryi Baker and Lilium regale Wilson were used as test materials and treated with different dose(0,2,4,6Gy) of ⁶⁰Co-γ rays. Statistical analyses of the germination rate, characters, physiological and biochemical indexes and variations.The results showed that the germination rate, plant height, bloomed rate and production of Lilium bosniacum, Lilium henryi Baker and Lilium regale Wilson gradually decreased with the increasing of radiation dosage. However, Lilium lancifolium radiated by 2 Gy showed no significant difference from the control,and by 4 Gy,the germination rate showed significant difference when compared with control. Under the radiation dose of 6 Gy,the seedballs could not sprout. 2 Gy dose radiation had little effect on the contents of chlorophyll for four varieties, 4 Gy treatment showed a downward trend.The activity of superoxide dismutase (SOD) increased first and then decreased in all varieties. While the malondialdehyde (MDA) increased gradually. The soluble sugar content of Lilium lancifolium Thunb increased first and then decreased, and other varieties increased with the increase of dose. The suitable radiation dose ranges for Lilium bosniacum, Lilium henryi Baker and Lilium regale Wilson were 2 to 4 Gy, 2 Gy and 2 Gy respectively. No appropriate dose was found for the Lilium lancifolium Thunb in this experiment. The results provide some technical references to a certain extent for Lilium radiation breeding.