紫外处理和热激处理调控芥菜芽苗主要生理生化及褪黑素富集研究

为研究紫外处理和热激处理对芥菜芽苗生理生化及其褪黑素富集的调控机制,本试验以芥菜籽粒为试材,研究经紫外处理和热激处理芥菜芽苗的主要生理生化指标及褪黑素合成关键酶基因表达情况。结果表明,经紫外处理和热激处理后,发芽期间,芥菜芽苗的芽长显著低于对照组(CK),可溶性蛋白含量和总抗氧化能力均显著高于CK(P<0.05),在观察时间点(4 和7 d),热激处理发芽4 d的芥菜芽苗褪黑素含量最高,分别是CK和紫外处理的2.89和2.47倍。发芽期间,紫外处理和热激处理各关键酶基因表达量存在显著差异,热激处理4 d时芥菜芽苗TDC2、T5H2及SNAT4的表达量较CK均显著上调,而SNAT2及ASMT3的表达量显著下调(P<0.05)。本研究表明热激处理可作为芥菜芽苗富集褪黑素的有效手段。

Regulation of Ultraviolet and Heat Treatments Regulates on the Main Physio-Biochemical and Melatonin Enrichment in Mustard Sprouts

In order to reveal the regulation mechanisms of ultraviolet and heat treatments on the physio-biochemical regulation and melatonin enrichment in mustard, mustard seeds were used as the test material, and physio-biochemical technology and fluorescence quantitative PCR were used to study the main physio-biochemical indexes and the expression of key enzyme genes of mustard sprouts under ultraviolet and heat treatments during germination. The results showed that the length of mustard sprouts under ultraviolet and heat treatments was significantly lower than that of in the control group(CK) during germination. Soluble protein content, and the total antioxidant capacity were significantly higher than that of the control treatment(P<0.05). At the time point of observation (4 d and 7 d), melatonin content was highest in the sprouts treated with heat for 4 days, which was 2.89 times of the control treatment and 2.47 times of ultraviolet treatment. On the 4th day of germination, compared with the control treatment, the expressions of TDC2, T5H2 and SNAT4 of the sprouts treated with heat were significantly up-regulated, while SNAT2 and ASMT3 significantly down-regulated (P<0.05). The results indicated that heat treatment could be an effective method to enrich melatonin in mustard sprouts.