iTRAQ蛋白质组学分析揭示西瓜响应CGMMV胁迫的蛋白质

黄瓜绿斑驳花叶病毒病(CGMMV)是一种危害西瓜生产的检疫性病害.为了在蛋白质水平研究西瓜对CGMMV的胁迫应答,本研究采用同位素标记相对和绝对定量(iTRAQ)技术对CGMMV接种前后的西瓜叶片进行蛋白质组学分析,获得参与CGMMV胁迫应答的差异表达蛋白质(DEPs),并对DEPs进行KEGG功能分析;利用Cytos...

iTRAQ-Based Proteomic Analysis Reveal Proteins Responsive to Cucumber Green Mottle Mosaic Virus Infection in Watermelon

Cucumber green mottle mosaic virus (CGMMV) is a quarantine disease that affects watermelon production. To investigate the CGMMV-associated defense response in watermelon at proteomic level, we sampled watermelon leaves before and post CGMMV inoculation and employed isobaric tag for relative and absolute quantification (iTRAQ) technology to identify the differentially expressed proteins (DEPs), and KEGG analysis was carried out for these DEPs. The protein-protein interaction (PPI) networks were constructed using Cytoscape software and RT-qPCR analysis were carried out for determining the expression patterns of proteins in response to CGMMV stress. In total, we quantified 61 proteins that differentially expressed in response to CGMMV stress. These differentially expressed proteins (DEPs) included ribosomal proteins, PSI and PSⅡ proteins, ATP synthase, NADH dehydrogenase, HSP22.8, eukaryotic translation initiation factor, sucrose synthase and ethylene response factor. They were involved in KEGG pathways of photosynthesis, biosynthesis of scondary metabolites, and plant hormone signal transduction. PPI analysis identified 13, 32 and 43 DEPs for 48 h_0 h, 25 d_0 h and 25 d_48 h that involved in the known PPI network under CGMMV stress. RT-qPCR analysis obtained the expression patterns of SUS4, RPL22, NAD7, PSBH, defensin-like protein 5, ATPF, LSP1 and HSP22.8 at different infection stages of CGMMV. These results identify the proteins’ responsive to CGMMV infection, and lay a foundation for revealing molecular mechanism of CGMMV resistance in watermelon.