甘草生长素反应因子(ARF)基因家族的鉴定及表达分析

本研究旨在鉴定甘草ARF基因家族并分析其在非生物胁迫下的表达模式。以乌拉尔甘草(Glycyrrhiza uralensis Fisch.)为研究对象,利用生物信息学方法对ARF基因家族进行鉴定,并对其蛋白质理化性质、保守结构域、基因结构、进化关系、顺式作用元件和表达模式分析。鉴定得到10个甘草ARF基因。其蛋白氨基酸序列长度在301~945 aa之间,分子量约为33.07~104.37kDa,等电点为5.93~8.50。亚细胞定位分析显示甘草ARF蛋白均位于细胞核。保守结构域分析显示GuARF蛋白大多包含B3、Auxin_resp和Aux/IAA结构域。基因结构发现外显子数量从6个到22个不等。在甘草ARF基因启动子区还存在5类不同的顺式调控元件。系统进化分析表明甘草ARF蛋白分为3类。转录组数据分析显示,10个GuARF基因在非生物胁迫下具有一定表达特异性。上述结果显示甘草ARF基因家族可能参与多种生物过程,这为进一步研究植物对非生物胁迫的生理和分子反应提供了有价值的信息。

Auxin Response Factor (ARF) Gene Family in Glycyrrhiza uralensis Fisch.: Identification and Expression Analysis

The purpose of the study is to identify the auxin response factor (ARF) gene family in Glycyrrhiza uralensis Fisch. and analyze their expression patterns under abiotic stress. G. Uralensis Fisch. was taken as the object. We identified the ARF gene family by bioinformatics tools, and analyzed the physicochemical properties of proteins, conserved motifs, gene structure, evolutionary relationships, cis-elements and expression profiles. 10 ARF genes (GuARFs) were identified. The length of these ARF proteins ranges from 301 to 945 amino acids and the relative molecular weight varies from 33.07 to 104.37 kDa, with PIs in the range of 5.93 to 8.50. Subcellular localization assays shows that the GuARF proteins are located in the nucleus. The conserved domain analysis indicates that most of GuARF proteins contain B3, Auxin_resp and Aux/IAA domain. Gene structure analysis reveals that the exon number of GuARF genes ranges from 6 to 22. There are 5 different types of cis-elements in the promoter region of GuARF genes. Phylogenetic analysis reveals that the GuARF proteins are clustered into 3 groups. RNA-seq data show that the expression level of 10 GuARF genes is different under abiotic stresses. The results indicate that the GuARF gene family may involve in multiple biological processes, and the study could provide valuable information for further research on physiological and molecular response of plants to abiotic stresses.