金花葵染色体倍性分析及属间远缘杂交研究

为了研究'金花葵3号'的染色体倍性,以'金花葵3号'新生根尖为材料,采用常规压片法,对'金花葵3号'进行染色体倍性分析,显示金花葵为二倍体,共含有66条染色体,核型公式为K(2n)=2x=66.以'金花葵3号'和含有52条染色体的异源四倍体陆地棉'邯818'为父母本进行杂交,并进行反交,结果显示:以'金花葵3号'为母本...

Chromosome Ploidy Analysis and Interspecific Hybridization of Abelmoschus manihot

To study the chromosome ploidy of ‘Abelmoschus manihot No.3’, using the new root tip of ‘Abelmoschus manihot No.3’ as material, the chromosome ploidy analysis was carried out by conventional squash method. The results showed that ‘Abelmoschus manihot No.3’ was diploid with 66 chromosomes, and the karyotype formula was K (2n)=2x=66. The hybrid F₁ could be obtained by crossing ‘Abelmoschus manihot No.3’ and allotetraploid upland cotton ‘Han 818’ with 52 chromosomes. The results showed that the hybrid F₁ could be obtained from the cross combination with ‘Abelmoschus manihot No.3’ as female parent. The PCR amplification results showed that the hybrid contained parental specific bands. The hybrid could develop normally, but the pollen was abortive and could not bear. All the young bolls of the hybrid combinations with ‘Abelmoschus manihot No.3’ as male parent fell off, and no hybrid was obtained. The results of squash showed that there were 59 chromosomes in the new root tip of F₁ hybrid, which further confirmed that F₁ hybrid was obtained by crossing parents. This study revealed for the first time the genetic regularity of chromosome ploidy and intergeneric hybridization in this species, which could provide a theoretical basis for further study on cytogenetics of Abelmoschus manihot .