14C-吡虫啉在苗期油菜中的吸收、转运与分布特性

为深入了解吡虫啉在植物体内的行为规律,本研究以14C-吡虫啉为研究对象,采用种衣剂拌种法,设低药种比(4.8和5.4 g ? kg-1)以及高药种比(6.0和7.2 g·kg-1)处理,利用同位素示踪技术及现代仪器分析技术,探讨了吡虫啉在苗期甘蓝型油菜中的动态吸收变化和转运分布规律.结果表明,药种比6.0 g·kg-1...

Uptake,Translocation and Distribution of 14C-Imidacloprid in Rape Seedlings (Brassica napus L.)

In order to further understand the behavior of imidacloprid in plants, uptake, translocation and distribution of ¹⁴C-Imidacloprid (¹⁴C-IMI) in rape seedlings were investigated using isotope tracer method and modern instrumental analysis technology in this study. The experiments were performed by seed-coating treatment with low IMI-seed ratios (4.8 and 5.4 g·kg^-1) and high IMI-seed ratios (6.0 and 7.2 g·kg^-1). The main results were as follows: Uptake percentage of IMI treated with IMI-seed ratio of 6.0 g·kg^-1 was the highest in rape seedlings among all treatments. The content of IMI in rape seedlings with the high IMI-seed ratios was significantly higher than that of the low IMI-seed ratio groups (P<0.05). Furthermore, no significant difference was found between T₃ and T₄, with the content of IMI in rape seedlings maintaining in the range of 4.72~7.96 and 4.20~9.42 mg·kg^-1 respectively. For low IMI-seed ratio groups, the content of IMI in the stem of the plants was significantly higher than that in other parts of the plants, and the IMI content of the oldest leaf (leaf 1) was higher than other leaves. The regular pattern on the distribution of ¹⁴C-IMI in the plant was stem > leaf > root. However, for high IMI-seed ratio groups, the content of IMI in stem and leaf 1 was significantly higher than that in other parts, and the ¹⁴C-IMI distribution pattern was found to be leaf >stem > root. The distribution ratio of ¹⁴C-IMI in the roots was in the range of 9.81%~16.37%; IMI was more accumulated in leaf margins. Transport factors (TF) of IMI were over 1 in all experimental seedlings and decreased with the extension of incubation time. In summary, the IMI-seed ratio of 6.0 g·kg^-1 would be suggested for processing rapeseeds. The results of this study provide essential data for guiding the scientific use of IMI in agricultural production and safety evaluation of IMI.