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套作白三叶草对香蕉枯萎病发病率及土壤微生物群落的影响
引用本文:刘满意,王禹童,孙铭泽,李荣,王蓓蓓. 套作白三叶草对香蕉枯萎病发病率及土壤微生物群落的影响[J]. 热带生物学报, 2021, 12(2): 219-227. DOI: 10.15886/j.cnki.rdswxb.2021.02.011
作者姓名:刘满意  王禹童  孙铭泽  李荣  王蓓蓓
作者单位:海南大学热带作物学院,海口 570228;南京农业大学资源与环境科学学院,南京 210095
基金项目:国家自然科学基金项目(41867006)
摘    要:为了探明套作白三叶草(Trifolium repens L)后连作蕉园土壤微生物群落的变化及对香蕉枯萎病发病率的影响,在连作蕉园中套作白三叶草,设置单作香蕉和套作白三叶草处理,并通过高通量测序的方法研究了不同处理间土壤微生物群落结构和组成的差异.结果表明,与单作香蕉处理相比,套作白三叶草处理将香蕉枯萎病发病率降低了13...

关 键 词:高通量测序  香蕉枯萎病  尖孢镰刀菌  微生物群落
收稿时间:2020-12-22

Effect of Intercropping of Banana with White Clover on Banana Fusarium Wilt and Soil Microbial Community
Affiliation:1.College of Tropical Crops, Hainan University, Haikou, Hainan 570228, China2.College of Resource and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China
Abstract:An attempt was made to explore the changes of soil microbial community in continuous cropping banana orchards after banana was intercropped with white clover (Trifolium repens L) and the effect of the intercropping on the incidence of banana Fusarium wilt. Banana was intercropped with white clover in continuous cropping banana orchards, with monoculture of banana as control. The soil microbial community structure and composition among different treatments in the banana orchards were detected by using high-throughput sequencing and their differences were analyzed. Compared with the monoculture of banana, intercropping of banana with white clover reduced the disease incidence of banana Fusarium wilt by 13.34%, increased the pH value by 0.54, and increased the content of soil available potassium by 7.21 mg·kg?1. Compared with the monoculture of banana, the intercropping of banana with white clover significantly increased soil microbial diversity, and formed a unique community structure. Moreover, the intercropping of banana with white clover changed the community composition of soil microorganisms, significantly decreased the relative abundance of Fusarium oxysporum, and increased the relative abundance of GP16 and Davidiella. It is concluded that the intercropping of banana with white clover in continuous cropping banana orchards significantly reduced the number of F. oxysporum, increased soil pH and microbial diversity, changed the community structure of microorganisms, and then reduced the incidence of banana Fusarium wilt disease.
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