超声辅助提取陕产天麻多糖的工艺优化及抗氧化活性研究

旨在优化商洛天麻多糖的提取工艺并分析其抗氧化活性。本研究以商洛市天麻为材料,通过正交试验对超声辅助热水浸提法提取天麻多糖的工艺进行优化,并测定其DPPH自由基和羟基自由基的清除能力,以分析其抗氧化活性。结果表明,超声波辅助热水浸提法提取天麻多糖的最佳工艺条件为提取温度65℃,提取时间45 min,料液比1:40 g/mL,该条件下商洛天麻多糖含量为32.83 mg/g。天麻多糖清除DPPH自由基及羟基自由基的能力随浓度的增大而升高,说明天麻多糖含量在一定范围内有良好的清除DPPH自由基及羟基自由基的效果。当浓度3.5 mg/mL时,天麻多糖对DPPH自由基清除率能达到40.52%,羟基自由基的清除率能达到36.52%。天麻多糖具有开发成抗氧化剂的潜力。

Gastrodia elata Polysaccharide from Shaanxi by Ultrasound-assisted Hot Water Extraction: Process Optimization and Antioxidant Activity

The aim of this study is to optimize the extraction process of polysaccharide of Gastrodia elata and to analyze its antioxidant activity. In this study, Gastrodia elata in Shanluo City was used as material, the technology of ultrasonic assisted hot water extraction for polysaccharide of Gastrodia elata was optimized by orthogonal test, and the scavenging ability of DPPH free radical and hydroxyl free radical was determined and the antioxidant activity was analyzed. The optimum extraction condition was: 65℃ for 45 min with a solvent-to-solid ratio of 40:1 g/mL. The polysaccharide yield of Gastrodia elata was 32.83 mg/g under the condition. The ability of polysaccharides in Gastrodia elata to scavenge DPPH and hydroxyl radicals was improved with the increase of concentration, indicating that polysaccharides in Gastrodia elata had excellent scavenging effect on DPPH and hydroxyl radicals within a certain range. When the concentration was 3.5 mg/mL, the DPPH scavenging rate of Gastrodia elata polysaccharide reached 40.52% and hydroxyl radical was up to 36.52%. Polysaccharides from Gastrodia elata exhibit the potential to be developed into antioxidants.