| 马铃薯种质资源晚疫病抗性评价及分子标记辅助筛选 |
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| 引用本文： | 娄树宝,李凤云,田国奎,王海艳,田振东,王立春,刘喜才,王辉. 马铃薯种质资源晚疫病抗性评价及分子标记辅助筛选[J]. 作物杂志, 2021, 37(4): 196-100. DOI: 10.16035/j.issn.1001-7283.2021.04.030 |
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| 作者姓名： | 娄树宝 李凤云 田国奎 王海艳 田振东 王立春 刘喜才 王辉 |
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| 作者单位： | 1黑龙江省农业科学院克山分院/农业农村部马铃薯生物学与遗传育种重点实验室,161005,黑龙江齐齐哈尔2华中农业大学/农业农村部马铃薯生物学与生物技术重点实验室,430070,湖北武汉 |
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| 基金项目： | 黑龙江省农业科学院青年基金(2019YYYF017);国家自然科学基金“基于抗病基因组学和效应子识别策略的马铃薯晚疫病抗性优异基因资源鉴定、新抗病基因发掘与利用”(31761143007) |
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| 摘 要： | 由致病疫霉（Phytophthora infestans）引起的晚疫病是马铃薯生产上最具危害性的病害,种植抗病品种是防治该病害最有效的方法。分子标记辅助选择可以大大缩短育种年限,提高育种效率。对395008.45×克新27号的60份杂交后代进行了晚疫病抗性评价,结果有39份表现抗病,21份表现感病。利用晚疫病抗性基因R8的分子标记对60份杂交后代进行了检测,其中有43份含有R8基因;对36份种质资源进行检测,其中有28份含有R8基因。本研究鉴定的抗性资源可为马铃薯抗病育种提供优质的亲本材料。
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| 关 键 词： | 马铃薯 晚疫病 分子标记 |
| 收稿时间： | 2020-10-27 |
Evaluation of Germplasms for Resistance to Potato Late Blight and Molecular Markers Assisted Screening |
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| Lou Shubao,Li Fengyun,Tian Guokui,Wang Haiyan,Tian Zhendong,Wang Lichun,Liu Xicai,Wang Hui. Evaluation of Germplasms for Resistance to Potato Late Blight and Molecular Markers Assisted Screening[J]. Crops, 2021, 37(4): 196-100. DOI: 10.16035/j.issn.1001-7283.2021.04.030 |
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| Authors: | Lou Shubao Li Fengyun Tian Guokui Wang Haiyan Tian Zhendong Wang Lichun Liu Xicai Wang Hui |
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| Affiliation: | 1Keshan Branch of Heilongjiang Academy of Agricultural Sciences/Key Laboratory of Potato Biology and Genetics, Ministry of Agriculture and Rural Affairs, Qiqihar 161005, Heilongjiang, China2Huazhong Agricultural University/Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs, Wuhan 430070, Hubei, China |
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| Abstract: | Potato late blight caused by Photophthora infestans has always been a devastating disease in potato production. Use of resistant varieties is the most fundamental and effective way to control the disease. Molecular marker assisted selection can greatly shorten the breeding period, significantly improve the breeding efficiency. A total of 60 offspring of hybrid combinations of 395008.45×Kexin No.27 were evaluated for resistance to Phytophthora infestans. The results showed that 39 materials were resistantance varieties and 21 materials were susceptible varieties. The molecular marker related to the resistance gene R8 were used to detect 60 offspring and 36 potato germplasm resources, and 43 varieties (lines) and 28 varieties (lines) had resistance gene R8, respectively. The potato resistance germplasm resources tested could provide high-quality parental materials for disease resistance breeding. |
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| Keywords: | Potato Late blight Molecular markers |
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