基于高效液相色谱-质谱联用技术解析山栏稻营养品质的代谢物谱

以10份山栏稻（Oryza sativa L.）的种子为材料，利用超高效液相色谱?高分辨质谱联用技术进行山栏稻种子代谢数据库的建立，并对不同类型的山栏稻种子及种子不同萌发时期的代谢物进行定量分析，旨在为山栏稻营养品质的改良提供优良亲本和丰富水稻育种的种质资源。本研究成功构建了包含1 058个代谢信号的山栏稻种子代谢数据库，其中，氨基酸、脂质、维生素和类黄酮等已知物质645个。与普通水稻种子代谢谱的比较分析表明，山栏稻种子中不饱和脂肪酸、维生素等物质的相对含量高于普通水稻；甘油磷脂和黄酮类物质低于普通水稻。在种子萌发过程中，山栏稻与普通水稻种子中代谢物的积累模式存在差异，其中，脯氨酸、亚麻酸乙酯、泛酸和吡哆醇的积累模式在山栏稻和普通水稻中存在差异。

Analysis of the Metabolic Profile of the Nutritional Quality of Shanlan Upland Rice Based on High Performance Liquid Chromatography-mass Spectrometry

Seeds of 10 accessions of Shanlan upland rice were determined by using ultra-performance liquid chromatography-high resolution mass spectrometry to establish a Shanlan upland rice seed metabolism database, based on which the metabolites of the seeds of different types of Shanlan upland rice before germination and at different germination stages were analyzed by performing quantitative analysis. A database for Shanlan upland rice seed metabolism containing 1 058 metabolic signals was constructed, including 645 known substances such as amino acids, lipids, vitaminsvitamins, and flavonoids. Comparison and analysis of the metabolic profile of the seeds of Shanlan upland rice and conventional rice found that the relative contents of unsaturated fatty acids, vitamins and other substances were higher in Shanlan upland rice seeds than in the conventional rice seeds.T while the contents of glycerophospholipids and flavonoids were lower in the Shanland upland rice seeds than in the conventional rice. During seed germination, there were differences in the accumulation patterns of metabolites in the seeds of the Shanlan upland rice and the conventional rice, especially in the accumulation of proline, ethyl linolenate, pantothenic acid and pyridoxine.