不同产地林下三叶青多糖指纹图谱研究

建立三叶青多糖指纹图谱评价体系,对三叶青进行来源鉴别和质量控制。以不同产地三叶青为材料,用PMP柱前衍生化-HPLC法和化学计量学分析,测定三叶青多糖图谱及其单糖含量和组成比例。建立了不同产地三叶青多糖图谱及其共有模式,并确定了葡萄糖、鼠李糖、阿拉伯糖等8个单糖色谱峰。各批样品之间相似度较高,在0.934~0.997之间。根据主成分因子得分发现遂昌的多糖质量最好,温州的次之。经测定其单糖组成,得到甘露糖、葡萄糖、半乳糖和阿拉伯糖的含量较高,分别为9.094%、11.451%、56.695%和11.030%,其中半乳糖是三叶青多糖的主要组成成分。本研究建立了三叶青多糖指纹图谱并测定出其中单糖组成及其含量,该方法稳定可靠,可用于三叶青的来源鉴别和质量控制。

Under-forest Tetrastigma hemsleyanum: Polysaccharide Fingerprint in Different Producing Areas

The evaluation system of polysaccharide fingerprint was established to identify the source and control the quality of Tetrastigma hemsleyanum. T. hemsleyanum from different areas were used as materials, the polysaccharide spectrum, monosaccharide content and composition of T. hemsleyanum from different areas were determined by PMP pre-column derivatization-HPLC and chemometric analysis. The polysaccharide fingerprint and its common pattern were established for different origins, and 8 monosaccharide chromatographic peaks such as glucose, rhamnose and arabinose were determined. The similarity of each batch of samples was high, ranging from 0.934 to 0.997. According to the score of principal component factor, the quality of polysaccharide from Suichang was the best, followed by that from Wenzhou. The composition of its monosaccharides was determined, and high content of mannose, glucose, galactose and arabinose were obtained, which was 9.094%, 11.451%, 56.695% and 11.030%, respectively, with galactose being the main component of the polysaccharide of T. hemsleyanum. In this study, we established the polysaccharides fingerprint of T. hemsleyanum and determined the composition and content of monosaccharides. The method is stable and reliable, and can be used for the source identification and quality control of T. hemsleyanum.