基因型、施氮量及其互作对烤烟多酚类物质的影响

为探讨基因型、施氮量及其互作效应对烤烟多酚类物质含量的影响,采用二因素随机区组设计,以烤烟品种中烟100、云烟87和K326为材料,设置低氮（45kg/hm²）、中氮（60kg/hm²）和高氮（75kg/hm²）处理,研究其对烟叶中总酚、绿原酸、芸香苷和莨菪亭含量的影响。结果表明,基因型、施氮量及其互作效应对烤烟总酚、绿原酸、芸香苷和莨菪亭含量变异的贡献率不同,其中基因型对四者的贡献率分别为2.50%、5.13%、4.70%和15.95%,施氮量的贡献率分别为59.30%、42.55%、38.30%和67.79%,基因型与施氮量互作效应的贡献率分别为36.40%、51.20%、51.50%和15.03%。基因型与施氮量互作效应结果表明,高氮与中烟100互作条件下总酚、绿原酸和芸香苷含量较高,与K326互作时总酚和芸香苷含量较高,与云烟87互作时莨菪亭含量较高;中氮与云烟87互作时总酚、绿原酸和芸香苷含量较高,与K326互作时总酚、芸香苷和莨菪亭含量较高;低氮与云烟87互作时绿原酸含量较高。与种植品种相适应的施氮量是提高烟叶多酚类物质含量的重要措施。

Effects of Genotype,Nitrogen Application Rate and Their Interactions on Polyphenols in Flue-Cured Tobacco

To explore the effects of genotypes, nitrogen application rates, and their interactions on flue-cured tobacco polyphenols, a two-factor randomized block experiment was conducted with three flue-cured tobacco varieties, Zhongyan 100, Yunyan 87, and K326, and three nitrogen levels, low nitrogen (45kg/ha), medium nitrogen (60kg/ha) and high nitrogen (75kg/ha) was used as treatments to study their effects on the contents of total phenols, chlorogenic acid, rutin and scopoletin in tobacco leaves. The results showed that genotypes, nitrogen application rates, and their interactions contributed differently to the variation of flue-cured tobacco total phenols, chlorogenic acid, rutin, and scopoletin. The contribution rates of genotypes were 2.50%, 5.13%, 4.70% and 15.95%; The contribution rates of nitrogen application rates were 59.30%, 42.55%, 38.30%, and 67.79%; genotype and nitrogen application rate interaction were 36.40%, 51.20%, 51.50%, and 15.03%. The results of the interaction effect between genotype and nitrogen application rate showed that the contents of total phenols, chlorogenic acid, and rutin were higher under the conditions of high nitrogen and Zhongyan 100, and the contents of total phenols and rutin of K326 were higher. The contents of total phenols, chlorogenic acid, and rutin of Yunyan 87 were higher under medium nitrogen conditions, while those of total phenols, rutin, and scopoletin were higher when interacting with K326. The chlorogenic acid content of Yunyan 87 was higher under the condition of low nitrogen, . It is an important measure to increase the content of polyphenols in tobacco leaves by applying suitable nitrogen application rates for planting varieties.